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His-ATG3-Y210A

Plasmid

#202780

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Y210A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K208D

Plasmid

#202785

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K208D

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-C264A

Plasmid

#202778

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

C264A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-R265A

Plasmid

#202783

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

R265A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-H266A

Plasmid

#202784

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H266A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-NeoCOVGT3-d2EGFP-mCherry

Plasmid

#201450

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT3_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT1-d2EGFP-mCherry

Plasmid

#201451

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT1_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT2-d2EGFP-mCherry

Plasmid

#201452

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT2_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT4-d2EGFP-mCherry

Plasmid

#201453

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT4_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-IGFBP2-Clover

Plasmid

#191007

Purpose

Gateway entry vector with IGFBP2 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Gateway entry vector

Tags

Clover

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)

Plasmid

#180029

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ control-FLAG

Plasmid

#199678

Purpose

Bacterial expression of Leucine Zipper (LZ) control-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper control

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-Cterm-V5His6_H

Plasmid

#146488

Purpose

Insect Expression of DmPABPC1-Cterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-Cterm (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-Nterm-V5His6_H

Plasmid

#146489

Purpose

Insect Expression of DmPABPC1-Nterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-Nterm (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-Nterm-V5His6_H

Plasmid

#146478

Purpose

Insect Expression of DmPABPC1-Nterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-Nterm (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182_1-830-dsRNAres_G

Plasmid

#146388

Purpose

Insect Expression of DmGW182_1-830-dsRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182_1-830-dsRNAres (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182_1-1115-dsRNAres_G

Plasmid

#146389

Purpose

Insect Expression of DmGW182_1-1115-dsRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182_1-1115-dsRNAres (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S

Plasmid

#147461

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres-shRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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