We narrowed to 168,274 results for: Gene
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AEB-No-aptamer-control -- AEB3mRFP1
Plasmid#63851PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromoterAEB3Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pTC441
Plasmid#91218Purposeprotoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRExpressionPlantAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC437
Plasmid#91219Purposeprotoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRExpressionPlantAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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gabra5_R (TAL3015)
Plasmid#36007DepositorInsertTAL array targeting gabra5 (gabra5 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianAvailable SinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
gabra5_L (TAL3014)
Plasmid#36006DepositorInsertTAL array targeting gabra5 (gabra5 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianAvailable SinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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Human NCKX3 (pcDNA3.1+)
Plasmid#75205PurposeMammalian expression of SLC24A3DepositorAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWPT-UCU/ACC-mEGFP-IRES-mCherry
Plasmid#49232PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR 19b-3p
Plasmid#82442PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3pDepositorInserthsa-miR-19-3p
MutationComplementary region for hsa-miR-19-3p as sensorAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFusX4-31
Plasmid#63440PurposeEncodes RVD positions 10-12 in TALEN assemblyDepositorInsertHD NG NN
UseTALENMutationnoneAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only
