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pDONR223_IRF4_p.K123R

Plasmid

#81427

Purpose

Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway Cloning

Mutation

K123R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WIPI1_p.P139S

Plasmid

#81415

Purpose

Gateway Donor vector containing WIPI1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WIPI1 (WIPI1 Human)

Use

Gateway Cloning

Mutation

T31I; P139S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3IP1_p.G185E

Plasmid

#81339

Purpose

Gateway Donor vector containing PIK3IP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway Cloning

Mutation

G185E; T251S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_WT

Plasmid

#82096

Purpose

Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARAF (ARAF Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NBPF15_WT

Plasmid

#82068

Purpose

Gateway Donor vector containing NBPF15 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NBPF15 (NBPF15 Human)

Use

Gateway Cloning

Mutation

D477A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBP-TL4

Plasmid

#72988

Purpose

RBS_H5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

H5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL8

Plasmid

#72992

Purpose

RBS_L1

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pJK633

Plasmid

#72397

Purpose

Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)

Depositor

T. J. Kappock

Insert

phosphoribosylaminoimidazole synthetase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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His-TEV-U_(+36GFP)-Cre

Plasmid

#71750

Purpose

His-TEV-U+36GFP-Cre for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6X His and TEV protease

Expression

Bacterial

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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RHEBL1

Plasmid

#55684

Purpose

expression of mouse RHEBL1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RHEBL1 (Rhebl1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPRJi1

Plasmid

#59608

Purpose

Expression of shRNA against human PTPRJ

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRJ (PTPRJ Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pCB116

Plasmid

#53365

Purpose

To express Cup9p‐FLAG from the PGAL1 promoter

Depositor

Alexander Varshavsky

Article

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

Cup9 (CUP9 Budding Yeast)

Tags

Flag

Expression

Yeast

Promoter

GAL1

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pICH78141

Plasmid

#50293

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (Oryza sativa)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Tas2r3

Plasmid

#48176

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of TAS2R3 (TAS2R3 Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Kdr

Plasmid

#48178

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of 2KDR (MC2R Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pSG5-myc-p110α-CAAX

Plasmid

#39494

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Use

Eukaryotic

Tags

CAAX and myc

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
K-p22

Plasmid

#38787

Depositor

David Wang

Article

Siebrasse et al J Virol. 2012 Jun 27.

Insert

2545-3841 of MWPyV

Expression

Bacterial

Mutation

5 SNPs in comparison to WD976

Promoter

lac

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
K-p16

Plasmid

#38786

Depositor

David Wang

Article

Siebrasse et al J Virol. 2012 Jun 27.

Insert

214-1420 of MWPyV

Expression

Bacterial

Mutation

3 SNPs compared to WD976

Promoter

lac

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pCB210

Plasmid

#31722

Purpose

To express Cup9p‐FLAG from the PCUP9 promoter

Depositor

Alexander Varshavsky

Article

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

CUP9 (CUP9 Budding Yeast)

Tags

Cup9 promoter and FLAG

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only