We narrowed to 11,688 results for: nts

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pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

Plasmid

#215281

Purpose

Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Grin1 gRNA

Use

AAV

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH3.1-GS3

Plasmid

#204762

Purpose

Expression of Cas9 and human H3.1

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H3.1 (H3C6 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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HA-C Sars-CoV-2 E P71L

Plasmid

#194804

Purpose

Sars-CoV-2 E_P71L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

E_P71L (E Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P71L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-C Sars-CoV-2 N_T205I

Plasmid

#194812

Purpose

Sars-CoV-2 N_T205I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_T205I (N Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

T205I

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en1dFnCas9GFP

Plasmid

#201950

Purpose

Expression of en1dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en1dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1369R on FnCas9

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en15dFnCas9GFP

Plasmid

#201951

Purpose

Expression of en15dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en15dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1603H on FnCas9

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Plasmid

#202064

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT2

Plasmid

#210550

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM

Expression

Plant

Mutation

1bp insertion after AAVS1 target sequence that di…

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_PWP1:1-501

Plasmid

#210974

Purpose

Mammalian expression of full-length human PWP1. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PWP1:M1-S501 (PWP1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

E165D F448L G449R not annotated variants

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_PWP1:1-501

Plasmid

#210975

Purpose

Mammalian expression of full-length human PWP1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PWP1:M1-S501 (PWP1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

E165D F448L G449R not annotated variants

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-AtWUSSTOP

Plasmid

#202053

Purpose

E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

AtWUS E. Coli Codon Optimized (WUS Mustard Weed)

Tags

6xHis and GFP11

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2

Plasmid

#202661

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BPTI-21

Plasmid

#195058

Purpose

simplyfied amino acid sequence by alanine scanning

Depositor

Peter Kim, Yutaka Kuroda

Article

Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

Insert

bovine pancreatic trypsin inhibitor

Tags

His tag and trpΔLE leader sequences

Expression

Bacterial

Mutation

21 amino acids are Alanine out of 58 amino acids

Promoter

T7 promotor

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-ε

Plasmid

#197261

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-4 ε (AP4E1 Human)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

silent substitutions in codons 905, 1129 and 1137

Promoter

ADH1

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO

Plasmid

#192382

Purpose

To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Act2::FRT-OCS-FRT::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut1

Plasmid

#180316

Purpose

mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple-CAAX

Plasmid

#180324

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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