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pCTCON2-GFP-TAG

Plasmid

#158131

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Mutation

Introduced a stop codon in place of the tyrosine …

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_RUSC1-1/1

Plasmid

#91379

Purpose

Protein expression and purification of human SH3 domain construct RUSC1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RUSC1-1/1 (RUSC1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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TaRGET-ABE-C3.1 ver4.0

Plasmid

#188250

Purpose

Hypercompact adenine base editors

Depositor

Yong-Sam Kim

Article

Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

Insert

TaRGET-ABE3.1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN2-1/1

Plasmid

#91330

Purpose

Protein expression and purification of human SH3 domain construct PACSIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN2-1/1 (PACSIN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-XPLN

Plasmid

#73375

Purpose

GST-tagged expression of XPLN (ARHGEF3) in bacteria

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

GST

Expression

Bacterial

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-Barkor

Plasmid

#24402

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pESC-LEU-PmCDA1

Plasmid

#60818

Purpose

to overexpress lamprey deaminase in yeast

Depositor

Youri Pavlov

Article

Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

Insert

CDA1

Tags

FLAG

Expression

Yeast

Promoter

GAL10

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-5/5

Plasmid

#91464

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-5/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-5/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-1/5

Plasmid

#91351

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-1/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-3/5

Plasmid

#91352

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-3/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eYFP-f

Plasmid

#104057

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP-f

Use

AAV

Promoter

TRE

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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3xFNLDD/pMXs-puro

Plasmid

#49536

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFNLDD

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Promoter

LTR

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-HA-dSpCas9

Plasmid

#92112

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9

Use

CRISPR

Tags

HA tag and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pY006 (pcDNA3.1-hBpCpf1)

Plasmid

#69978

Purpose

Expresses humanized BpCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hBpCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pEY22

Plasmid

#191028

Purpose

lim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lim-4 (lim-4 Nematode)

Expression

Worm

Mutation

None

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY16

Plasmid

#191022

Purpose

flp-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-6 (flp-6 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY18

Plasmid

#191024

Purpose

flp-19 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-19 (flp-19 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY19

Plasmid

#191025

Purpose

flp-26 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-26 (flp-26 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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