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DDX3X - G123E in pENTR

Plasmid

#216574

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

G123E

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - I107V in pENTR

Plasmid

#216573

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

I107V

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (TEVp)

Plasmid

#213407

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

HA-arrestin-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi1-6

Plasmid

#197705

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi1 promoter

Expression

Plant

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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mTB3-FLAG tag

Plasmid

#210204

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

FLAG-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mTB3-AviD tag

Plasmid

#210203

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

AviD-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ300

Plasmid

#205414

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

Depositor

Yiping Qi

Article

Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

Insert

Ev1Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS (N terminal on insert) NLS (C terminal on ins…

Expression

Plant

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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nUnaG-ST

Plasmid

#207630

Purpose

A plasmid encoding the N-terminal UnaG fragment fused to SpyTag.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

nUnaG-SpyTag-MBP

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-84

Promoter

T7

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-BMPR2a

Plasmid

#207616

Purpose

Zebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

BMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Zebrafish, Vaucheria frigida)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCK389

Plasmid

#206791

Purpose

Sp.dCas9, pTet.MCP-SoxS, J23119.gRNA

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Inserts

dCas9
MCP-SoxS
scRNA

Promoter

J23119 and Tet

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3-NLS

Plasmid

#203489

Purpose

Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Gateway entry vector

Tags

Nuclear localization signal and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-MTS-jAspSnFR3-mRuby3

Plasmid

#203490

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Gateway entry vector

Tags

His-tag, Mitochondrial localization signal, and m…

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0019_T2c_BCD_0.1K

Plasmid

#200057

Purpose

Bicistronic RBS 0.1K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.1K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0028_T2c_BCD_18K

Plasmid

#200048

Purpose

Bicistronic RBS 18K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 18K

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0021_T2c_BCD_0.45K

Plasmid

#200055

Purpose

Bicistronic RBS 0.45K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.45K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0020_T2c_BCD_0.2K

Plasmid

#200056

Purpose

Bicistronic RBS 0.2K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.2K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0026_T2c_BCD_8.5K

Plasmid

#200050

Purpose

Bicistronic RBS 8.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 8.5K

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0023_T2c_BCD_1.5K

Plasmid

#200053

Purpose

Bicistronic RBS 1.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 1.5K

Expression

Bacterial

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0027_T2c_BCD_13K

Plasmid

#200049

Purpose

Bicistronic RBS 13K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 13K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0025_T2c_BCD_4.6K

Plasmid

#200051

Purpose

Bicistronic RBS 4.6K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 4.6K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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