170,342 results
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Plasmid#24609DepositorAvailable SinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits
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MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENExpressionMammalianAvailable SinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TBK1
Plasmid#23851DepositorInsertTBK1 (TBK1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE
Plasmid#140087PurposeBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistanceDepositorInsertLbCpf1 (LbCas12a) crRNA backbone
UseLentiviralTagsEF1a-PuroExpressionMammalianPromoterU6Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8s-WPRE
Plasmid#179256PurposeAAV-mediated expression of GCaMP8s under the CAG promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVExpressionMammalianPromoterCAGAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
Plasmid#47550PurposeCas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion alleleDepositorInsertsCas9
ttTi5605 sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available SinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-hIFITM2
Plasmid#58398PurposeExpressed human IFITM2 with an N-terminal HA tag in mammalian cellsDepositorAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_jGCaMP8m
Plasmid#213918PurposeAAV construct expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_jGCaMP8mDepositorHas ServiceAAV1InsertjGCaMP8m
UseAAVAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneTagsHA-Twin-StrepExpressionPlantPromoter2 X 35SAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSA091 PSMD14_IVS2-Cas9-BFP
Plasmid#249141PurposeOverexpression of SpCas9-BFP with PSMD14 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.DepositorInsertPSMD14_IVS2-Cas9-TagBFP (PSMD14 S. pyogenes Cas9, synthetic, Human)
UseCRISPR; Cp36 donor dnaExpressionMammalianMutationPSMD14 IVS2 intronPromoterEF1aAvailable SinceJan. 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-XNS
Plasmid#196655PurposePromega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directionsDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rInsp-hIns-GLuc
Plasmid#89927PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Synthetic, Human)
UseLentiviralExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoter410bp rat insulin II promoterAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER-CanlonicSF
Plasmid#178343PurposeTo explore endoplasmic reticulum lactate dynamicsDepositorInsertER-CanlonicSF
TagsER Retention Signal, ER Signal Exon 1, and ER Sig…ExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCL-HA-MAPKK1-8E (K97M)
Plasmid#40811DepositorAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
CSII-prEF1a-E2-Crimson-NLS
Plasmid#125263PurposeFluorescent reporter for cell sizeDepositorInsertE2-Crimson-NLS
UseLentiviralTagsNLSExpressionMammalianPromoterEF1aAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaSpCas9-NLS-6xHis
Plasmid#207386PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698A, amino acids 1005-1013…PromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-hHDAC3
Plasmid#98591Purpose3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.DepositorInsertdCAS9-HDAC3 (HDAC3 S. pyogenes, Synthetic, Human)
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
Plasmid#141391PurposeLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-N (N SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCB’ CbbL Y72R
Plasmid#162707PurposeCarboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomesDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutationCbbL Y72R; Substitution of CsoS2-binding tyrosine…PromoterPLtet0-1 promoterAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rrub-FII-prk
Plasmid#162695PurposeExpresses R. rubrum Form II rubisco and S. elongatus PrkDepositorInsertsRubrum Form II rubisco
S. elongatus PRK
ExpressionBacterialPromoterPLtet0-1 promoter and under same promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only