We narrowed to 22,943 results for: ARC;

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dimericTomato-1xwGBD

Plasmid

#176099

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt12

Plasmid

#184513

Purpose

Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 12 (Syt12 Rat)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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Cyto-RA

Plasmid

#209860

Purpose

Dimerization dependent fluorescent protein RA fused to NES

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

NES sequence NLVDLQKKLEELELDEQQ

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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miniCMV eGFP hPGK BSD

Plasmid

#188519

Purpose

Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Dsup pLVX-Puro

Plasmid

#180635

Purpose

Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

Depositor

Christopher Mason

Article

Westover et al bioRxiv 2020.11.10.373571

Insert

Dsup

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-mem-TurboID

Plasmid

#149413

Purpose

Hepatocyte-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAC1749

Plasmid

#127983

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pVH607-Tier1-PCREm4-Nluc

Plasmid

#169594

Purpose

Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDule-Tet3.0

Plasmid

#174080

Purpose

Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

Insert

Tet3.0 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

L270G, Y271L, N311G, C313A

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9

Plasmid

#188489

Purpose

Expresses FLAG tagged SpCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-tagged human full length TCF4E pcDNA3

Plasmid

#32738

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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dCas12j2 KRAB

Plasmid

#188508

Purpose

Expresses FLAG tagged dCas12j2 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt5

Plasmid

#184506

Purpose

Mammalian expression of Synaptotagmin 5 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 5 (Syt5 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-VGLUT2-pHluorin

Plasmid

#207469

Purpose

Lentiviral expression of VGLUT2-pHluorin

Depositor

Robert Edwards

Article

Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

Insert

VGLUT2 (Slc17a6 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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AsCas12a

Plasmid

#188492

Purpose

Expresses FLAG tagged AsCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-pT7-sfGFP_RED20

Plasmid

#135173

Purpose

Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

Depositor

Drew Endy

Article

Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

Insert

sfGFP_RED20

Use

Synthetic Biology

Mutation

Recoded ORF of sfGFP to only use one codon per am…

Promoter

taatacgactcactata

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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