Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 31,908 results for: fer

Showing: 10901 - 10920 of 31908 results

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Const.4 EGFP-IRES-Luc-PS9

Plasmid

#231908

Purpose

For packaging EGFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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mproSAAS (21 kDa)

Plasmid

#231634

Purpose

Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

Depositor

Iris Lindberg

Article

Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

Insert

Pcsk1N (Pcsk1n Mouse)

Expression

Mammalian

Mutation

Deletion of C-terminus (aa181-225)

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-dgat1a in situ probe

Plasmid

#232137

Purpose

zebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1a (dgat1a Zebrafish)

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(E502Q)

Plasmid

#226973

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(E502Q)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

E502Q

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pWR58

Plasmid

#202795

Purpose

integrating bioluminescene reporter for Cas9 activity in S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

P-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence

Expression

Yeast

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh13CDS

Plasmid

#192853

Purpose

Plasmid to produce ISH probe for mMyh13 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 13 (Myh13 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh13UTR

Plasmid

#192854

Purpose

Plasmid to produce ISH probe for mMyh13 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 13 (Myh13 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-FCPF

Plasmid

#220132

Purpose

Expresses Cas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

Cas9-FCPF (cas9 S. pyogenes)

Tags

His and NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD_beta-gamma

Plasmid

#226981

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD_beta-gamma

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392A)

Plasmid

#226980

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392F)

Plasmid

#226979

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392F)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392F

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(F401A)

Plasmid

#226978

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(F401A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

F401A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(L616E)

Plasmid

#226977

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(L616E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

L616E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226K)

Plasmid

#226976

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226K)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226K

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226M)

Plasmid

#226975

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226M)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226M

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226E)

Plasmid

#226974

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514E)

Plasmid

#226972

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514A)

Plasmid

#226971

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(C500S)

Plasmid

#226970

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(C500S)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

C500S

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(G853A)

Plasmid

#226969

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(G853A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

G853A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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