We narrowed to 24,423 results for: lars;

1. pGEX-4T-3-SH3BP1-NTD

   Plasmid

   #136648

   Purpose

   Expression of SH3BP1-NTD-GST fusion protein in E. coli

   Depositor

   Karl Matter, Maria Balda

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1-NTD (SH3BP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains only N-terminal domain

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEM-sgMUC4_dual-MTS-3x-Set I

   Plasmid

   #162762

   Purpose

   Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBTK102

   Plasmid

   #110574

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   T7 promoter with RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pWUR 1+2 tetO mut89

   Plasmid

   #80102

   Purpose

   Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

   Depositor

   George Church

   Article

   Shipman et al Science. 2016 Jun 9. pii: aaf1175.

   Inserts

   Cas1 (cas1 )
   Cas2 (cas2 )
   Cas1 (cas1 )
   Cas2 (cas2 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q24H, P202Q, G241D, E276D, L297Q

   Promoter

   Available since

   Dec. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFP.R400

   Plasmid

   #138175

   Purpose

   Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mStrawberry-Vimentin-7

   Plasmid

   #55945

   Purpose

   Localization: Intermediate Filaments, Excitation: 574, Emission: 596

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC-PHYT

   Plasmid

   #53300

   Purpose

   Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

   Insert

   crtE, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK_ToeRepG2_N02_trigger

   Plasmid

   #132745

   Purpose

   T7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNA

   Depositor

   Peng Yin, Alexander Green

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   ToeRepG2_N02_trigger
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME-AKT-PH

   Plasmid

   #67674

   Purpose

   Gateway entry clone containing AKT-PH with no stop codon

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   AKT-PH
   

   Use

   Gateway multisite middle entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BC-A1-002

    Plasmid

    #78689

    Purpose

    A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS::AtHAP2-YFP

    Plasmid

    #132960

    Purpose

    Express AtHAP2 full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    At HAP2::YFP
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTK7_013

    Plasmid

    #123880

    Purpose

    Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    RA_hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJL017

    Plasmid

    #53532

    Purpose

    This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpBA
    

    Use

    Tags

    6His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OMM PKA Reporter

    Plasmid

    #104550

    Purpose

    Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Tom20 - mTurquoise2 - VASP 148-164
    

    Use

    Tags

    mTurqouise2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH49

    Plasmid

    #128211

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKV016

    Plasmid

    #64767

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-NHERF1-PDZ2

    Plasmid

    #28296

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF1 (NHERF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains aa 148-298 (deletion of the first PDZ do…

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

18. TRAPa-3xFLAG-pCDNA

    Plasmid

    #201963

    Purpose

    Mammalian expression of Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK1_008

    Plasmid

    #123671

    Purpose

    Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-2xHS4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRTS-GBP-dCas9-mRFP

    Plasmid

    #102934

    Purpose

    Tetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

    Depositor

    Sebastian Bultmann

    Article

    Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GBP (GFP binding protein and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only