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R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

Plasmid

#159542

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12V

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Plasmid

#159562

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Plasmid

#159569

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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C098

Plasmid

#174345

Purpose

Template for making Nluc mRNA reporter with pA139.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Available Since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

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C034

Plasmid

#174340

Purpose

Template for making Rluc mRNA reporter with pA98.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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C033

Plasmid

#174339

Purpose

Template for making Rluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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C031

Plasmid

#174338

Purpose

Template for making Rluc mRNA reporter with pA30.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pXD69m1-TetW

Plasmid

#191246

Purpose

E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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B3-4

Plasmid

#111474

Purpose

translational release factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pXD69m2-AphA

Plasmid

#191247

Purpose

E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N20_switch

Plasmid

#132722

Purpose

T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N20_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N20_trigger

Plasmid

#132726

Purpose

T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N20_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-rpiL*

Plasmid

#90320

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS rpiL*

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKB233

Plasmid

#170040

Purpose

Encodes PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Insert

mNeonGreen

Use

Synthetic Biology

Expression

Bacterial

Promoter

PvirK

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm1

Plasmid

#99355

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm1

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-3

Plasmid

#90327

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJBM4

Plasmid

#225291

Purpose

E. coli-Campylobacter shuttle vector; TetR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Tet(O) and its putative promoter

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pIA1421

Plasmid

#172407

Purpose

Expression of SARS-COV-2 Nsp12 K50A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1405

Plasmid

#172404

Purpose

Expression of SARS-COV-2 Nsp12 D218A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D218A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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