159,461 results

1. pcDNA3-TDsmURFP-IRES-HO-1

   Plasmid

   #80346

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL20_mEGFP_NUP98_NSD1

   Plasmid

   #205873

   Purpose

   Express mEGFP-tagged fusion protein, NUP98_NSD1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NUP98_NSD1 (NUP98, NSD1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dendra2-CD9-10

   Plasmid

   #57705

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Insert

   CD9 (CD9 Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pUC19-hAPOE-R-loop

   Plasmid

   #134901

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   APOE promoter (APOE Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-hSNRPN-R-loop

   Plasmid

   #134902

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   hSNRPN promoter (SNRPN Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB117 - pL2_pSB90_fLUC-I_V2

   Plasmid

   #123192

   Purpose

   binary plant vector for transient TYLCV V2 and fLUC (with intron) expression

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::V2::tNOS 2x35S::fLUC-I::tNOS
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PS-mOrange2-MyosinIIB-C-18

   Plasmid

   #57812

   Purpose

   Localization: Cytoskeleton, Excitation: 548 / 634, Emission: 565 / 662

   Depositor

   Michael Davidson

   Insert

   MyosinIIB (MYH10 Human)
   

   Use

   Tags

   PS-mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLH-spsgRNA2

   Plasmid

   #64114

   Purpose

   Vector for expression of Sp sgRNA2 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp sgRNA2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92390

   Purpose

   expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHMR1A

    Plasmid

    #102957

    Purpose

    gene cloning of MR1A

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

    Insert

    MR1A cDNA (MR1 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Stop codon has been removed by HindIII site

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBlueKan+cysMPro

    Plasmid

    #187879

    Purpose

    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

    Depositor

    Gaylen Uhlich

    Article

    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CysM

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGST2-GST-TEV-OPTN

    Plasmid

    #198428

    Purpose

    Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPEY-GAL-Nat

    Plasmid

    #232097

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

    Plasmid

    #58434

    Purpose

    Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)4-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB1619 hU6-BpiI-Cas13bt1-DR

    Plasmid

    #176314

    Purpose

    hU6-BpiI-Cas13bt1-DR (crRNA expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    Cas13bt1 crRNA direct repeat
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. tph-1(short)::rpl-22-HA

    Plasmid

    #122203

    Purpose

    C. elegans tagged ribosome expression plasmid

    Depositor

    Steven Flavell

    Article

    Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

    Insert

    tph-1(short)::rpl-22-HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB1C3-Lux

    Plasmid

    #109383

    Purpose

    Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-LUX operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. UKNP2.4.1

    Plasmid

    #98212

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-Puro-Myr-Flag-IKBKE

    Plasmid

    #15293

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only