We narrowed to 7,491 results for: PAC;

1. pC002 - LshC2C2 locus into pACYC184

   Plasmid

   #79151

   Purpose

   Endogenous LshC2c2 locus cloned into pACYC184

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   LshC2c2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAC95-pmax-dCas9VP160-2A-neo

   Plasmid

   #48227

   Purpose

   dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
   

   Use

   CRISPR

   Tags

   2A-neo, HA Tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-pac-T2A-dre

   Plasmid

   #124833

   Purpose

   Puromycin resistance gene and dre recombinase

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-dre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAcGFP-Hyg-CTCF-ZnF-N1

   Plasmid

   #131802

   Purpose

   Expresses the [Zinc finger domain of human CTCF (a.a. 265-603)]-GFP in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-1731

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPBbsr-6080NES(Epac-sensor)

   Plasmid

   #162487

   Purpose

   hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Epac-sensor (RAPGEF3 Human, Synthetic)
   

   Use

   Tags

   YPet and Turquoise2GL

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

   Plasmid

   #216384

   Purpose

   Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Norrin (NDP Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C and Rho1D4

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

   Plasmid

   #79259

   Purpose

   Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   DmeIF4G (eIF4G1 Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   V5-His6 and lambdaN-HA

   Expression

   Mutation

   Promoter

   Ac5 promoter

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CaMKII-pAceR-Kv2.1PR

   Plasmid

   #195531

   Purpose

   Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAceR-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM 78E, 81D, 92N; SI linker

   Promoter

   CaMKII

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC5-dual-dCas9VP48-sgTetO

    Plasmid

    #48237

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48 and sgTetO
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcGFP-Hyg-CTCF-Nterm-N1

    Plasmid

    #131800

    Purpose

    Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 796-2181 (the N-terminus is l…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAcGFP-Hyg-CTCF-Cterm-N1

    Plasmid

    #131801

    Purpose

    Expresses the [C-terminus of human CTCF (a.a. 577-728)]-GFP in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-1731

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgRNA scaffold-spacer-human U6

    Plasmid

    #214697

    Purpose

    provide "sgRNA scaffold-spacer-human U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-human U6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid

    #183876

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAcGP67A-rCL1-HormR-GAIN

    Plasmid

    #37847

    Depositor

    Axel Brunger

    Article

    Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

    Insert

    CL1 (Adgrl1 Rat)
    

    Use

    Baculovirus transfer vectors

    Tags

    8xHIS

    Expression

    Mutation

    HormR and GAIN domains only (aa460–849

    Promoter

    Polyhedrin

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pACP-GPI Control Plasmid

    Plasmid

    #101131

    Purpose

    Expression of ACP-Tagged GPI anchor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    GPI
    

    Use

    Tags

    ACP-Tag (ACP wt)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAc5.1B-EGFP-DmGIGYF_641-1574_AH

    Plasmid

    #148920

    Purpose

    Insect Expression of DmGIGYF_641-1574

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_641-1574 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmGIGYF_1-640_AH

    Plasmid

    #148919

    Purpose

    Insect Expression of DmGIGYF_1-640

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_1-640 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFGC5941-PacI-ath-STTM2937

    Plasmid

    #84112

    Purpose

    target miRNA2937 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM2937
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACP-ADRβ2 Control Plasmid

    Plasmid

    #101128

    Purpose

    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Use

    Tags

    ACP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge