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pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC

Plasmid

#148414

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1318-1605-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA

Plasmid

#194367

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194368

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-AVPR2-NTEV-TCS-GV-2xHA

Plasmid

#194371

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_AVPR2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194372

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA

Plasmid

#194375

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194376

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHA

Plasmid

#194363

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD2-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194364

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut1

Plasmid

#18085

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut1 (SLC2A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Luc-N

Plasmid

#58790

Purpose

Express LucN alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 1-398

Use

Tags

FLAG

Expression

Mammalian

Mutation

T576C,T591C to destroy MfeI and EcoRI sites

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK4

Plasmid

#122675

Purpose

Expresses mouse PCSK4 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK7

Plasmid

#122676

Purpose

Expresses mouse PCSK7 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NINJ1

Plasmid

#201180

Purpose

Transient expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK2

Plasmid

#122673

Purpose

Expresses mouse PCSK2 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+DPP6

Plasmid

#117272

Purpose

Encodes human DPP6 variant 1

Depositor

Mark Cannell

Article

Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

Insert

DPP6 (DPP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-OGT_opt

Plasmid

#190858

Purpose

For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Use

Tags

Expression

Mammalian

Mutation

Codon optimized for expression in human cells.

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-AKAR2-CR

Plasmid

#40255

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

AKAR2

Use

Tags

His6 and Xpress

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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a5-ecto-pcDNA31hygro

Plasmid

#113386

Purpose

Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

Insert

human alpha 5 ectodomain

Use

Tags

Strep II tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b

Plasmid

#52122

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only