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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

Plasmid

#171090

Purpose

CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

Depositor

Sebastian Lourido

Article

Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

Inserts

Sth1 dCas9
Chloramphenicol acetyltransferase
TagBFP

Use

CRISPR; Toxoplasma gondii expression

Tags

3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

Expression

Mutation

D9A, H599A

Promoter

TUB1 (Toxoplasma gondii), transcriptionally linke…

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-NRR-T2A-BFP

Plasmid

#194929

Purpose

LV construct encoding NRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-NRR-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186F-T2A-BFP

Plasmid

#194931

Purpose

LV construct encoding Y186F mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186A-T2A-BFP

Plasmid

#194932

Purpose

LV construct encoding Y186A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

Plasmid

#156409

Purpose

pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

mutant KRAS4A-G12D

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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bactin-loxp-BFP-loxp-dsred-pBSII

Plasmid

#68972

Purpose

for transgenic animals

Depositor

David Traver

Article

Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

Inserts

lox-BFP-loxp
dsred

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

Plasmid

#31477

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 T2A mTagBFP

Use

Lentiviral

Tags

HA and NLS

Expression

Mutation

F13Y + S111Y on I-AniI = Y2 "reo" (res…

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187089

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Use

Tags

TagBFP-P2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

Plasmid

#187091

Purpose

GABA sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-iGABASnFR

Use

Tags

TagBFP-P2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-R103A-T2A-BFP

Plasmid

#194935

Purpose

LV construct encoding R103A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

Plasmid

#133328

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All F to S in CTD of full-length TDP43

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

Plasmid

#120268

Purpose

Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

Plasmid

#102594

Purpose

Lentivector to express STING V155M delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M delta 342

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

Plasmid

#71213

Purpose

dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A

Use

Tags

dSpCas9-BFP-DNMT3A(E752A)

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-BFP

Plasmid

#137130

Purpose

Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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