We narrowed to 12,964 results for: CAR

1. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

   Plasmid

   #171810

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 CARD8 FIIND F405G + S297A

   Plasmid

   #169974

   Purpose

   pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   S297A, F405G

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 CARD8 FIIND E274R + S297A

   Plasmid

   #169968

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   S297A, E274R

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 CARD8 FIIND R394E + S297A

   Plasmid

   #169972

   Purpose

   pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   S297A, R394E

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 LIC 6A CARD8-FLAG R394E

   Plasmid

   #169954

   Purpose

   Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R394E

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

   Plasmid

   #169958

   Purpose

   Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG and Ub

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 LIC 6A CARD8-FLAG L368G

   Plasmid

   #169952

   Purpose

   Mammalian expression of CARD8-FLAG with a site III mutation (L368G)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L368G

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 LIC 6A CARD8-FLAG E274R

   Plasmid

   #169950

   Purpose

   Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E274R

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A CARD8-FLAG F370G

   Plasmid

   #169953

   Purpose

   Mammalian expression of CARD8-FLAG with a site III mutation (F370G)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F370G

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB.His.MBP.3C NLRP1 UPA-CARD E1461R

    Plasmid

    #164007

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    E1461R

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB.His.MBP.3C NLRP1 UPA-CARD W1460A

    Plasmid

    #164006

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    W1460A

    Available Since

    Feb. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB.His.MBP.3C NLRP1 UPA-CARD M1457A

    Plasmid

    #164005

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    M1457A

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A

    Plasmid

    #164004

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Y1445A

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-Synapsin-mScarlet-optoJNKi-WPRE

    Plasmid

    #159968

    Purpose

    optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mScarlet-optoJNKi
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23-IL2RA CaRE2 (minus strand)

    Plasmid

    #91847

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0624)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE2 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs6602425 A>C, G>A mutation at chr10:611706…

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.23-CD69 CaRE1 (minus strand)

    Plasmid

    #91842

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0619)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE1 (minus strand) (CD69 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.23-IL2RA CaRE6 (minus strand)

    Plasmid

    #91840

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0617)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE6 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs7893467 C>A

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.23-IL2RA CaRE6 (plus strand)

    Plasmid

    #91839

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0616)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE6 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs7893467 G>T, rs11256448 A>G

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.23-IL2RA CaRE1 (minus strand)

    Plasmid

    #91838

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0615)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.23-CD69 CaRE2 (plus strand)

    Plasmid

    #91848

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0625)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE2 (plus strand) (CD69 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge