We narrowed to 2,111 results for: D10

1. pMLM3705

   Plasmid

   #47754

   Purpose

   Expresses mammalian cell codon-optimized dCas9-VP64

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   codon optimized dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9 (catalytically i…

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHSE901

   Plasmid

   #91708

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

   Plasmid

   #105235

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Mammalian_SpCas9_Target-AID3S

   Plasmid

   #188649

   Purpose

   Expresses SpCas9_Target-AID3S in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID3S-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SpCas9

   Promoter

   pCMV

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAN-PTet-dCas9

   Plasmid

   #62244

   Purpose

   controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   catalytically inactive, D10A, H840A

   Promoter

   Ptet

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. A3A-Gam

   Plasmid

   #119769

   Purpose

   Conversion of C to T monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
   

   Use

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_Blasto

   Plasmid

   #167864

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBa.Lamp1-GFP

   Plasmid

   #180250

   Purpose

   Mammalian expression of LAMP1

   Depositor

   Marvin Bentley

   Article

   Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Chicken Beta actin

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p35S-A3A-PBE

   Plasmid

   #119773

   Purpose

   Conversion of C to T in potato

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS-UGI-NLS
   

   Use

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   CaMv 2x35S

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnxABE-flgn

    Plasmid

    #187932

    Purpose

    A-to-G base editor

    Depositor

    Yi Xiao

    Article

    et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

    Insert

    ecTadA(8e)-nxCas9 (D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TAL2430

    Plasmid

    #36829

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HDAC2-TALEN_Left (Hdac2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pKin1A

    Plasmid

    #31607

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    kinesin-1A (Kif5a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p35S-PBE

    Plasmid

    #119772

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    NLS-APOBEC1-nCas9-UGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. NAX_CAGGS_CAS9_m4_Puro

    Plasmid

    #167871

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TAL2431

    Plasmid

    #36830

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HDAC2-TALEN_Right (Hdac2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. SlugBE4max

    Plasmid

    #163799

    Purpose

    Expresses AncBE4max and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. JEH127

    Plasmid

    #179299

    Purpose

    Expresses dCas9 fused to VPR

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCXLE-dCas9GFP-shP53

    Plasmid

    #102906

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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