We narrowed to 7,665 results for: tev

Showing: 1081 - 1100 of 7665 results

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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R701-X71-527: His6-tev-Hs.CALM1(1-80)

Plasmid

#159694

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(1-80)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(1-80) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-OPTN WT

Plasmid

#190191

Purpose

Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Tags

6xHis, TEV, and mCherry

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPB EF1a CCR6-eLOV-TEVcs-Gal4

Plasmid

#173119

Purpose

Expresses CCR6 eLOV receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-Gal4

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

Plasmid

#44094

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

Plasmid

#207355

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (2R-T)

Plasmid

#29717

Depositor

Scott Gradia

Type

Empty backbone

Tags

StrepII-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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His-TEV-Aurein1.2_(+36GFP)-LPETG-His

Plasmid

#71755

Purpose

His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-LPETG-His

Tags

6XHis, Sortase (LPETG), and TEV Protease

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)

Plasmid

#44082

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194377

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2 D597N

Plasmid

#136236

Purpose

Expressing catalytically inactive human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

Insert

human Argonaute-2 (AGO2 Human)

Tags

Sterp Sumo

Expression

Insect

Mutation

D597N

Promoter

polyhedrin

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

Plasmid

#194385

Purpose

Gateway Destination vector for split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

attR1-ORF-attR2-NTEV-TCS-GV-2xHA

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

Plasmid

#217896

Purpose

Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB5 (PSMB5 Human)
PSMB6 (PSMB6 Human)
PSMB7 (PSMB7 Human)

Tags

2xSTII

Expression

Insect

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

Plasmid

#139788

Purpose

Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Arneet Saltzman

Article

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCR6-eLOV-TEVcs-tTA

Plasmid

#173116

Purpose

Expresses CCR6 receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Promoter

Synapsin

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

Plasmid

#105801

Purpose

Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: FLAG-3C; ORF2: mClover3-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

Plasmid

#44081

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

12xHis tag, 6xGly linker, ADH1 transcription term…

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)

Plasmid

#44071

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

3xFlag, 6xGly linker, ADH1 transcription terminat…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

Plasmid

#105805

Purpose

Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-Cerulean; ORF2: TEV-Venus

Expression

Mammalian

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-dCas9-BirA*

Plasmid

#159994

Purpose

Bacterial expression of dCas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

dCas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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