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NCKX4 scFv [N414/25]

Plasmid

#190553

Purpose

Mammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-Venus-iGluSnFR.A184S

Plasmid

#106195

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Tags

Expression

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

GFAP

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Synaptotagmin-6 scFv [N270/47]

Plasmid

#190544

Purpose

Mammalian Expression of Synaptotagmin-6 scFV. Derived from hybridoma N270/47.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Synaptotagmin-6 (Mus musculus) recombinant scFV (Syt6 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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TrpV1 scFv [N221/17]

Plasmid

#182082

Purpose

Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pmiR-report-MYB-WT

Plasmid

#141114

Purpose

Reporter plasmid of WT MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

CMYB (MYB Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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TrpC5 scFv [N67/15]

Plasmid

#190564

Purpose

Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

Plasmid

#106179

Purpose

Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

Promoter

hSynapsin

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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Rufy3 scFv [N483/26]

Plasmid

#190558

Purpose

Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TrpV1 scFv [N221/17]

Plasmid

#190540

Purpose

Mammalian Expression of TrpV1 scFV. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpV1 (Rattus norvegicus) recombinant scFV (Trpv1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiR-report-MYB-mut

Plasmid

#141115

Purpose

Reporter plasmid of mutant MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

A to T mutations at m6A sites

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-Venus-iGluSnFR.A184V

Plasmid

#106196

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

GFAP

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-Venus-iGluSnFR.S72A

Plasmid

#106197

Purpose

Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

Promoter

GFAP

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSOX17-T2A-dTom-PGK-Neo

Plasmid

#223190

Purpose

Tomato reporter plasmid targeting the human SOX17 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

SOX17-T2A-dTomato-PGK-Neo-SOX17 (SOX17 Human)

Use

Cre/Lox; Human targeting

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFOXA2-T2A-mTagBFP2-PGK-Neo

Plasmid

#223191

Purpose

BFP reporter plasmid targeting the human FOXA2 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)

Use

Cre/Lox; Human targeting

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL5ET-12.EGFP.BCC6.WPRE

Plasmid

#229638

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

Jing et al bioRxiv

Insert

RMacL5ET-12.EGFP.BCC6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL3-05.EGFP.BCB2.WPRE

Plasmid

#229620

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

Jing et al bioRxiv

Insert

RMacL3-05.EGFP.BCB2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL3-07.EGFP.BCB4.WPRE

Plasmid

#229622

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

Jing et al bioRxiv

Insert

RMacL3-07.EGFP.BCB4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL3-09.EGFP.BCB7.WPRE

Plasmid

#229624

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

Jing et al bioRxiv

Insert

RMacL3-09.EGFP.BCB7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL3-10.EGFP.BCB8.WPRE

Plasmid

#229625

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

Jing et al bioRxiv

Insert

RMacL3-10.EGFP.BCB8

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL3-11.EGFP.BCB12.WPRE

Plasmid

#229626

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

Jing et al bioRxiv

Insert

RMacL3-11.EGFP.BCB12

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge