We narrowed to 2,596 results for: ubiquitin

1. CAV1-K*R-mEGFP

   Plasmid

   #27766

   Depositor

   Ari Helenius

   Article

   Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

   Insert

   caveolin-1, lysine null mutant (CAV1 Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   All lysines mutated to arginines (K*R)

   Promoter

   Available since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. XE251 pCDNA3.1(zeo)-hDsh3

   Plasmid

   #16758

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3 (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-KLF5-FLAG

   Plasmid

   #40917

   Depositor

   Jin-Tang Dong

   Article

   Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

   Insert

   Kruppel-like factor 5 (KLF5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. sAB-MBP

   Plasmid

   #204736

   Purpose

   synthetic antigen-binding fragment specifically recognizing bacteria maltose-binding protein

   Depositor

   Minglei Zhao

   Article

   Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

   Insert

   synthetic antigen-binding fragment recognizing bacteria maltose-binding protein
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-p62-LIR-mutant (LIRAAAA)

   Plasmid

   #187981

   Purpose

   Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   p62 (NUP62 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   DDDW335-338AAAA (LIR mutant), silent mutation siR…

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. M76 pIRESpuro-GLUE-hDsh2

   Plasmid

   #15101

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   Dsh2 (DVL2 Human)
   

   Use

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO-FNTA-FLAG

   Plasmid

   #86858

   Purpose

   To express FNTA with a FLAG tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTA (FNTA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The sequence of the insert is isoform 2, which di…

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBMN-2xFKBP-mEGFP-NDP52

   Plasmid

   #110465

   Purpose

   retrovirus construct for stable expressing mEGFP tagged NDP52

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Retroviral; Retrovirus

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-eCas9-sgMIB1

    Plasmid

    #140239

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    sgRNA targeting human MIB1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVhPOP1_3Flag

    Plasmid

    #53968

    Purpose

    Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    POP1 (POP1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

    Plasmid

    #226350

    Purpose

    Expression of proteasome substrate with tail for molecular ruler

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET151-HIS-TEV-GST-PP-RPN13

    Plasmid

    #73741

    Purpose

    Expression of hRPN13 in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    6xHIS and GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-HA-KLF5-his

    Plasmid

    #40904

    Depositor

    Jin-Tang Dong

    Article

    Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

    Insert

    Kruppel-like factor 5 (KLF5 Human)
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI-neo-Myc-Rabex-5

    Plasmid

    #196937

    Purpose

    Expresses Myc-tagged Rabex-5 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    Rabex-5 (RABGEF1 Bovine)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CAV1-K*R-HA

    Plasmid

    #27765

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1, lysine null mutant (CAV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    All lysines mutated to arginines (K*R)

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3-myc3-FBW5

    Plasmid

    #19905

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    F-box and WD repeat domain containing 5 (FBXW5 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSFDuet1-His-TEV-OTC_Ub

    Plasmid

    #172134

    Purpose

    Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

    Depositor

    Shang-Te Danny Hsu

    Article

    Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

    Insert

    E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA SF-HERC2 F4

    Plasmid

    #55787

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. 3xFLAG-CUL7-pCMV7.1

    Plasmid

    #155025

    Purpose

    N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 7 (CUL7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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