We narrowed to 88,518 results for: MAL

1. pLgw EcoDam-V5-ELK1

   Plasmid

   #98604

   Purpose

   Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

   Plasmid

   #196890

   Purpose

   Neuron-specific expression of human (h)Centrin fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_sgFLNB-SK2

   Plasmid

   #119873

   Purpose

   gRNA expression vector

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgFLNB-SK2 (FLNB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_sgFLNB-SK4

   Plasmid

   #119874

   Purpose

   gRNA expression vector

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgFLNB-SK4 (FLNB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

   Plasmid

   #118412

   Purpose

   Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

   Depositor

   Nicole Paulk

   Article

   Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

   Insert

   Firefly luciferase
   

   Use

   AAV, Luciferase, and Synthetic Biology

   Expression

   Insect and Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

   Plasmid

   #198569

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPc1 hp47phox (S303/304/328D)

   Plasmid

   #112245

   Purpose

   expression of mutant p47phox

   Depositor

   Simone Di Giovanni

   Article

   Hervera et al Nat Cell Biol. 2018 Mar;20(3):307-319. d

   Insert

   p47phox (NCF1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S303/304/328D

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139097

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sgHcn1#2/Cre

   Plasmid

   #193218

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgHcn1#2 (Hcn1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid

    #136652

    Purpose

    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

    Plasmid

    #71213

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
    

    Tags

    dSpCas9-BFP-DNMT3A(E752A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-td-mCherry-Cdk5rap2

    Plasmid

    #196859

    Purpose

    Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187949

    Purpose

    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-ZIP(RK)

    Plasmid

    #27136

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Mutation

    Arginines in the SH2 domains mutated to lysines (…

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINDgw Cbx1 V5 EcoDam

    Plasmid

    #59203

    Purpose

    Mammalian DamID positive control plasmid. Expresses Cbx1-V5-Dam.

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Insert

    Cbx1 (Cbx1 Mouse)
    

    Use

    Damid

    Tags

    Dam and V5

    Expression

    Mammalian

    Promoter

    heat shock minimal pomoter

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307_PDHA2S293A

    Plasmid

    #115197

    Purpose

    Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    p.S293A

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. iMb-Control-Mosaic (IR98.10)

    Plasmid

    #99748

    Purpose

    Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    MbYFP, MbTomato, MbKate2
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO_GFP_AAVS1

    Plasmid

    #141211

    Purpose

    pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDECKO_mCherry_AAVS1_1801

    Plasmid

    #159090

    Purpose

    targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF2255

    Plasmid

    #141994

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNTL (BMAL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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