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pET His10-Draper delta ITAM

Plasmid

#126671

Purpose

Expression of His10-Draper delta ITAM mutant for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic ITAM residues mutated to Phe

Expression

Bacterial

Mutation

Cytoplasmic ITAM residues mutated to Phe

Availability

Academic Institutions and Nonprofits only

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NaVSp1 FL DeltaCTD pIRES

Plasmid

#128077

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel. Residues 1-224.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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NaVAe1 FL DeltaCTD pIRES

Plasmid

#128078

Purpose

Mammalian cell expression vector for NaVAe1 bacterial sodium channel. Residues 1-241.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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NaVAe1 DeltaCC pIRES

Plasmid

#128086

Purpose

Mammalian cell expression vector for NaVAe1 1-264 chimera bacterial sodium channel.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

Plasmid

#117979

Purpose

SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1-B2

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-HIS-ybbr

Plasmid

#117980

Purpose

SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

Plasmid

#117983

Purpose

SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B2

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pLV-eGFP

Plasmid

#36083

Purpose

3rd generation lentiviral transfer plasmid

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-NEUROD1-T2A-mCherry

Plasmid

#178582

Purpose

Expression of NEUROD1 and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pKL2299A

Plasmid

#222105

Purpose

A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

Depositor

Kan Wang

Article

Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

Insert

virA

Expression

Bacterial and Plant

Promoter

virA promoter

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM37443

Plasmid

#153218

Purpose

Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPY22-mNeonGreen-Hyg

Plasmid

#137082

Purpose

Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

Depositor

Gohta Goshima

Article

Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

Insert

mNeonGreen-Flag

Expression

Plant

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[TEV]

Plasmid

#200122

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-WP1-CRY2PHR-VP64

Plasmid

#171758

Purpose

Lentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

WP1-CRY2PHR-VP64

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Hsp90 HA

Plasmid

#22487

Depositor

William Sessa

Article

Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

Insert

Hsp90 HA (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pegfp-N1-TFEB-S3A,R4A

Plasmid

#44446

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed both Serine 3 and Arginine 4 to Alanine

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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