We narrowed to 22,456 results for: his
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pIA1421
Plasmid#172407PurposeExpression of SARS-COV-2 Nsp12 K50A in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - C2 (UCSF)
Plasmid#169209PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCjedCas9
Plasmid#172216PurposeExpresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cellsDepositorInsertcatalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
TagsHistagMutationchanged Asp 8 to Ala and His 559 to AlaAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF7a
Plasmid#169393PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFPDepositorInsertSARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_NSP16
Plasmid#169389PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFPDepositorInsertSARS-COV-2 _NSP16
UseCell-free expression vectorTagsHis and eGFPMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_NSP9
Plasmid#169383PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP9 with N-term eGFPDepositorInsertSARS-COV-2 _NSP9
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_M
Plasmid#169400PurposeCell-free/mammalian expression vector for SARS-COV-2 virus M with N-term eGFPDepositorInsertSARS-COV-2 _M (M SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBPcyto-TEV-BseRI
Plasmid#157739Purposeempty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneTagsHis10 + MBP (cytoplasmic expression; no signal se…ExpressionBacterialPromoterT7Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS32: pTwinStrep-SUMO-PmcTnsC
Plasmid#168165PurposePmcTnsC expression construct for protein purification.DepositorInsertPmcCAST TnsC
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10
Plasmid#162385PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
Tags6xHisExpressionInsectMutationQ315LPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-RARS-5
Plasmid#166535PurposescFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-KARS-5
Plasmid#166530PurposescFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-TTc-4xDQNAT
Plasmid#128401PurposePeriplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertTetanus toxin fragment C domain
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
Tags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb66
Plasmid#159422PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66DepositorInsertSb66
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Z-AIMP1-1
Plasmid#166560PurposescFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only
