We narrowed to 24,953 results for: ina
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Plasmid#1191DepositorPublicationInsertN-terminus of Sup35 (SUP35 Budding Yeast)
UseCup1 promoterTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGRE NPD-GFP
Plasmid#1197DepositorPublicationInsertN-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP11-LRRK2_G2019S
Plasmid#231175PurposeExpresses GFP-11-LRRK2 G2019S in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP11-LRRK2 (G2019S) (LRRK2 Human)
TagsGFP11ExpressionMammalianMutationG2019SPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TMEM16F-M522K-peGFP-N1
Plasmid#228579PurposeExpresses mouse TMEM16F-M522K-eGFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
ExpressionMammalianMutationM522K plus a 3 amino acid N-terminal truncation (…Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCH62 (denAsCas12a-KRAB piggyBac)
Plasmid#217333PurposepiggyBac expression of denAsCas12a-KRABDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdenAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationD908A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shABC1
Plasmid#186713PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.DepositorAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MARK4_IDG-K
Plasmid#189884PurposeGateway destination clone of MARK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertMARK4 (MARK4 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG657 H2B-pFAST
Plasmid#172862PurposeExpresses pFAST fused to zebrafish histone H2B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-pFAST
TagsMyc tag (C terminal on insert)ExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA NC
Plasmid#176259PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI andDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseMutationH840A and D10A mutations on spCas9 to inactivate …Available sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT18
Plasmid#170906PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV superYFP-AURKA-mTurq2
Plasmid#157772PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAURKA (AURKA Human)
TagsmTurquoise2 and superYFPExpressionMammalianPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Archon1-KGC-GFP-ER2
Plasmid#115890PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianPromoterEF1a(1.1kb short version)Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI-NLS. PGK-puro
Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4133721
Plasmid#152946PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Arf6 T27N- Venus in pcDNA3.1
Plasmid#90464PurposeRhoA mediated cell migrationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArf6 T27N (ARF6 )
TagsVenusExpressionMammalianPromoterCMVAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mIRFP670nuc-TEV-Xph20-ETWV
Plasmid#133044PurposeExpresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertXph20-ETWV
TagsNLS, TEV protease + TEV cleavage site, and miRFP6…ExpressionMammalianMutationS63KPromoterCAGAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
Plasmid#122471PurposeDox-inducible GFP (N-terminal tag) SCAF4 expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSCAF4 (SCAF4 Human)
TagsGFPExpressionMammalianMutationSynonymous mutations conferring resistance to gRN…PromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-ExRai-AKAR-Kras
Plasmid#118408PurposePlasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExRai-AKAR-Kras
TagsC-terminal targeting sequence from KrasExpressionMammalianPromoterCMVAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113688PurposeSaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only