We narrowed to 11,688 results for: nts
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Plasmid#146213PurposeBacterial Expression of HsEDC3_192-228-HsRck-K135ADepositorInsertHsEDC3_192-228-HsRck-K135A (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E
Plasmid#146215PurposeBacterial Expression of HsEDC3_192-508-HsRck-E236QDepositorInsertHsEDC3_192-508-HsRck-E236Q (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E
Plasmid#146216PurposeBacterial Expression of HsEDC3_192-508-HsRck-K135ADepositorInsertHsEDC3_192-508-HsRck-K135A (EDC3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjT-tdTomato-PGK-Neo
Plasmid#186173PurposeKnock-in gene targeting in marmoset cells at TBXT locusDepositorInserttdTomato
UseMarmoset targetingAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-tdTomato-PGK-Neo
Plasmid#186172PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserttdTomato
UseMarmoset targetingAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK-N328A
Plasmid#115246PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tagDepositorInsertNODAL splice variant (NODAL Human)
TagsMYC-DYKExpressionMammalianMutationN328APromoterCMVAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_W403A_6xH
Plasmid#185712PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
Tags6xHis and MBPExpressionBacterialMutationChanged W403 to APromoterT7Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
3020_pETcon-SARS-CoV-2-RBD_N501Y
Plasmid#184405Purposeyeast surface display of the SARS-CoV-2 Alpha variant RBDDepositorInsertSARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationN501YAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-N501Y-A570D-AVI
Plasmid#176449PurposeExpresses SARS-CoV-2 RBD-SD1 domain containing N501Y-A570D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-SD1-N501Y-A570D (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationN501Y, A570DAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-L452R-E484Q-AVI
Plasmid#176455PurposeExpresses SARS-CoV-2 RBD-SD1 domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-SD1-L452R-E484Q (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationL452R, E484QAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-B.1.526-S477N-AVI
Plasmid#176329PurposeExpresses SARS-CoV-2 S2P protein from the B.1.526 variant containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.429 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT95I, D253G, S477N, D614G, A701VAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-B.1.526-E484K-AVI
Plasmid#176330PurposeExpresses SARS-CoV-2 S2P protein from the B.1.526 variant containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.429 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT95I, D253G, E484K, D614G, A701VAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-B.1.617.1-AVI
Plasmid#176333PurposeExpresses SARS-CoV-2 S2P protein from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.617.1 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q…Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (P.1 lineage / Brazilian variant)
Plasmid#169847PurposeMammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tagDepositorInsertProtein S
TagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (B.1.1.7 lineage / UK variant)
Plasmid#169848PurposeMammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tagDepositorInsertProtein S
TagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p35S_EVDdi
Plasmid#167120PurposePlant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.DepositorInsertEVADE (EVD) (AT5G17125 Mustard Weed)
ExpressionPlantMutationDeleted intron from full length elementPromoterCaMV 35SAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA E4-En-1 (GB2243)
Plasmid#160565PurposetRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInserttRNA-gRNA position E4-En-1 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)
Plasmid#126758PurposeExpression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)DepositorInsertevoSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationM495V, Y515N, K526E, R661QPromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-B-eSpCas9
Plasmid#126772PurposeExpression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)
Plasmid#126753PurposeExpression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)DepositorInsertWT SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only