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plentiCRISPR-sgGOT1

Plasmid

#72874

Purpose

The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgGOT1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pNC953

Plasmid

#20402

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP-reporter

Expression

Yeast

Mutation

X=UbiE (see schematic) linker=deltak

Available Since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-deadMalibu-mScarlet

Plasmid

#229555

Purpose

Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

Depositor

Danielle Schmitt

Article

Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

Insert

Malibu-mScarlet

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Circular-oc-EMCV-GFP

Plasmid

#226261

Purpose

Production of in vitro circularizable RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pR562

Plasmid

#207886

Purpose

Creation of Level 0 entry clones

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

amilCP

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJLG039

Plasmid

#192982

Purpose

BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

Depositor

Clay Fuqua

Article

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

T7 polymerase

Expression

Bacterial

Promoter

lac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRN3P_AID-Dam-only

Plasmid

#136065

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam only

Use

In vitro transcription

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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iTol2Amp-γ-crystallin:RFP

Plasmid

#108455

Purpose

This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

Tol2-Amp-y-cryst-mCherry

Use

Gateway Cloning

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.944

Plasmid

#108523

Purpose

PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCin-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.713

Plasmid

#108514

Purpose

PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PLuxB-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO pTRE

Plasmid

#68457

Purpose

TOPO construct expressing pTRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Tetracycline Response Element Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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R5 MCS

Plasmid

#51732

Purpose

Dicistronic vector with cloning site

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

MCS

Promoter

SV40

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pNH7

Plasmid

#40858

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NH7

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pNH8

Plasmid

#40859

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NH8

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pNH10

Plasmid

#40861

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NH10

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pUC-mCA1-emptyMCS

Plasmid

#35095

Depositor

James Fleet

Article

Xue et al Mol Cancer Res. 2010 Aug;8(8):1095-104.

Type

Empty backbone

Use

Mouse Targeting

Promoter

mouse carbonic anhydrase 1

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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ptac-KanL94P

Plasmid

#15927

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

Kanamycin Resistance

Expression

Bacterial

Mutation

L94P

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pET21a-Med25-VBD-6His

Plasmid

#64773

Purpose

Bacterial expression of His-tagged Med25-VBD

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VBD (MED25 Human)

Expression

Bacterial

Mutation

insert is 394-543 only

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pET14b-PITPalpha

Plasmid

#58256

Purpose

bacterial expression of human PITP alpha

Depositor

Shamshad Cockcroft

Article

Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

Insert

PITP alpha (PITPNA Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pTipi2.1-AZU_SP

Plasmid

#229144

Purpose

Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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