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dmBACCS2NS-IRES-dOrai-IRES-GFP

Plasmid

#72895

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc3-FBW5 delta F

Plasmid

#19912

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 delta F (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Mutation

deleted aa1-42 (F-box)

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pHS1871

Plasmid

#205976

Purpose

U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH DR-BpiI-DR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1862

Plasmid

#205975

Purpose

FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5-His

Plasmid

#103785

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5-His

Tags

EYFP, His-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein promoter-less

Plasmid

#118007

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Plasmid

#105643

Purpose

Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

Depositor

Sandra Rieger

Article

Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

Insert

Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Expression

Mammalian

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only
pHS1517

Plasmid

#205980

Purpose

FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMP71-mIL15-IL12rb1-sushi-il12rb2

Plasmid

#174606

Purpose

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SpeCas9-HF

Plasmid

#192141

Purpose

Expresses highly specific SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9-HF

Use

AAV

Expression

Mammalian

Mutation

SpeCas9-HF (R247A, N415A, S421A)

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAV10.KN

Plasmid

#63209

Purpose

pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry-pBirA-HA

Plasmid

#154458

Purpose

expresses mCherry-pBirA-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry-pBirA-HA

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-JNKAR

Plasmid

#181857

Purpose

Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-JNKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-nsp7-FLAG

Plasmid

#201027

Purpose

Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

8xHis and FLAG

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pClneoMyc human MST2

Plasmid

#37022

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MST2 (STK3 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
FUW-ubiquitin-SV40-RFP

Plasmid

#65448

Purpose

Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-RFP

Use

Lentiviral

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
TIA1 RRM-YFP-SspBx6

Plasmid

#103781

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-YFP-SspBx6

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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