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pEGFP-PLAA

Plasmid

#85669

Purpose

Expression of PLAA with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

PLAA (PLAA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-p97-E578Q

Plasmid

#85672

Purpose

Expression of human p97 E578Q mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

p97 (Vcp Rat)

Tags

GFP

Expression

Mammalian

Mutation

E578Q

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

Plasmid

#211904

Purpose

Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

CD19-CAR-28z-2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

hPGK1

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT54

Plasmid

#218726

Purpose

Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

Insert

IFT54 (TRAF3IP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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Nanog-luciferase targeting construct

Plasmid

#26591

Depositor

Rudolf Jaenisch

Article

Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

Insert

Nanog promoter-luciferase-lox-pgk-neomycin-loc

Expression

Bacterial

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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attB‐mCh‐Poly(A)-PuroR

Plasmid

#65529

Purpose

PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCOG75-Sem1 Sc

Plasmid

#83233

Purpose

pCDF-His6-MBP-Sem1 Sc

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

SEM 1 (SEM1 Budding Yeast)

Tags

His-mbp

Expression

Bacterial

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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CP3

Plasmid

#223335

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-miniTurboID-EGFP-3HA

Tags

miniTurboID-EGFP-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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CP2

Plasmid

#223334

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-EGFP-TurboID-3HA

Tags

EGFP-TurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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CP1

Plasmid

#223333

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-TurboID-EGFP-3HA

Tags

TurboID-EGFP-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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aTY50_pPGK1_Pcad_IRES_PuroR_WPRE

Plasmid

#225348

Purpose

Lentiviral vector for Pcad expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Pcad (Cdh3 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CP4

Plasmid

#223336

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-EGFP-miniTurboID-3HA

Tags

EGFP-miniTurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-EGFP

Plasmid

#210318

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2

Plasmid

#69624

Purpose

A FRET sensor for Galphai2 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Promoter

CMV and CMV (IRES)

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

Plasmid

#69623

Purpose

A FRET sensor for Galphai1 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai-mTurquoise2

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Promoter

CMV and CMV (IRES)

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

Plasmid

#69625

Purpose

A FRET sensor for Galphai3 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Promoter

CMV and CMV (IRES)

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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