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Plasmid#100956PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only
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pJH2971
Plasmid#100955PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH2970
Plasmid#100954PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSCD44A-c002
Plasmid#175493PurposeProtein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.DepositorInsertSingle chain Fv CD44 antibody (H90)
UseTags3XFLAG-EK-His8 and pelBExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
FL-DYSF-pUC57
Plasmid#60177PurposePlasmid contains full-length human dysferlin in a multiple cloning site of pUC57-Kan and can be easily cloned into other vectors.DepositorInsertDysferlin (DYSF Human)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human Coxsackievirus A16 strain G10 inactive 2A protease
Plasmid#228665PurposeBacterial expression of the A71 2A protease, innactive and can be used as a control in assaysDepositorInsertEnterovirus A71 2A protease
UseTagsHis-SUMOExpressionBacterialMutationAvitag insertion and C2A mutations / Active site …PromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Clim Act MCH (CAM)
Plasmid#104443PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim EF1 MCH (CEM)
Plasmid#104444PurposeExpresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoterEF1Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Tub MCH (CTM)
Plasmid#104445PurposeExpresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromotertubulin promoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Flex-Axon-EGFP
Plasmid#135429PurposeCan be used to drive axon-enriched expression of EGFP in the presence of Cre recombinase.DepositorHas ServiceAAV1Insertaxon-EGFP
UseAAV and Cre/LoxTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSyn1Available sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIS0
Plasmid#12178Purpose3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein productionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-mScarlet-WPRE
Plasmid#99280PurposeCan be used to generate AAV virus that will express mScarlet in the presence of CreDepositorInsertmScarlet
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human-Dysferlin-v1
Plasmid#60217PurposeHuman dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (DYSF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-WGA-mCherry
Plasmid#224119PurposeTo express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.DepositorInsertWGA-mCherry
UseAAVTagsWGA and mCherryExpressionMammalianMutationAll mutations related to the WGA in this construc…PromoterCAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mouse-Dysferlin-Transcript-Variant1
Plasmid#60214PurposeMouse dysferlin transcript variant-1 (accession #NM_021469) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (Dysf Mouse)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-mRuby3-WPRE
Plasmid#99279PurposeCan be used to generate AAV virus that will express mRuby3 in the presence of CreDepositorInsertmRuby3
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse-Dysferlin-Transcript-Variant2
Plasmid#60215PurposeMouse dysferlin transcript variant-2 (accession #NM_001077694) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (Dysf Mouse)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3
Plasmid#107736PurposeIn the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.DepositorInsertsynaptophysin-GCaMP6s, mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoternEFAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c010
Plasmid#175482PurposeProtein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.DepositorInsertSDCBP (SDCBP Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(AscI)
Plasmid#15404DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutation700 bp insert can be released by digestion with r…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only