We narrowed to 1,806 results for: pDest

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GFP-D1a_W2-3(aa318-399)

Plasmid

#21525

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa318-399

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
GFP-D1a_W3-4(aa340-439)

Plasmid

#21526

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa340-439

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
GFP-D1a_W4-5(aa409-495)

Plasmid

#21527

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa409-495

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
Tol2-kdrl:mCherry-caax

Plasmid

#194287

Purpose

Expression plasmid for zebrafish or Danionella cerebrum

Depositor

Pui-Ying Lam

Article

Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

Insert

kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)

Use

Zebrafish or danionella cerebrum expression

Tags

mCherryCAAX from the Tol2kit (Kwan et al., 2007)

Promoter

kdrl(flk1)

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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p667-UBC-ZNF746-V5-miniTurbo

Plasmid

#207820

Purpose

C-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-V5-miniTurbo (ZNF746 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUAS5x:GFPNLS-SV40pA

Plasmid

#155122

Purpose

Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x:GFPNLS

Use

Zebrafish expression

Promoter

QUAS5x-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:GFPNLS-SV40pA

Plasmid

#155123

Purpose

Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-HA-CYLD

Plasmid

#22544

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

Plasmid

#191500

Purpose

GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol2 transgenes…

Promoter

myo6b

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-STAM1

Plasmid

#164628

Purpose

Expresses STAM1 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

STAM1 (STAM Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2021

Availability

Academic Institutions and Nonprofits only

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GST-Delta23C11orf83

Plasmid

#65849

Purpose

bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of the 23 first aa (TM)

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pJS145

Plasmid

#179159

Purpose

Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

Depositor

Iva Greenwald

Article

Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Insert

rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR

Expression

Worm

Mutation

Flexon replacing 2nd intron of 2xNLS::GFP

Promoter

rps-27p

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF3

Plasmid

#207815

Purpose

N-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF3 (NFE2L3 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF3-V5-miniTurbo

Plasmid

#207816

Purpose

C-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF3-V5-miniTurbo (NFE2L3 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dll4P1Egfp

Plasmid

#90160

Purpose

contains dll4 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4P1Egfp (dll4 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;lmo2P1Egfp

Plasmid

#90162

Purpose

contains lmo2 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3D+3N

Plasmid

#234614

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DN (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -4D+4N

Plasmid

#234611

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DN (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N, D262N

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -4D+4V

Plasmid

#234610

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DV (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V, D262V

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3D+3V

Plasmid

#234613

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DV (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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p667-UBC-MAFG-V5-miniTurbo

Plasmid

#207818

Purpose

C-terminal tagged MAFG for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

MAFG-V5-miniTurbo

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MAFG

Plasmid

#207817

Purpose

N-terminal tagged MAFG for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-MAFG

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746

Plasmid

#207819

Purpose

N-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

Plasmid

#198381

Purpose

Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

UAS:sypb-egfp-cry2

Use

Tol2

Tags

EGFP

Mutation

D387A

Promoter

UAS

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Ash1-5p

Plasmid

#169712

Purpose

Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

Depositor

Tanja Mittag

Article

Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

Insert

Ash1 (ASH1 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

Truncation including encoding amino acids 420-480…

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dusp5P1Egfp

Plasmid

#90161

Purpose

contains dusp5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D1aMut(W>A)

Plasmid

#21522

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503, mutate every tryptophan (total…

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
DF-UAS-spa-GCaMP6f

Plasmid

#67568

Purpose

Zebrafish expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

HIS-tag

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only