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pJH2217

Plasmid

#179251

Purpose

Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Psra-11

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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p1977 (pUC19-PTDH3-PTEF1)

Plasmid

#78230

Purpose

Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Bidirectional promoters pTDH3-pTEF1

Expression

Bacterial

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-BBS4

Plasmid

#218713

Purpose

Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS4 (BBS4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

I354T (t1110c)

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1b2

Plasmid

#13752

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b2 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pEG BacMam C term 3C eGFP myc

Plasmid

#160685

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-NRASlong

Plasmid

#13797

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

NRAS (NRAS Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk3a2

Plasmid

#15748

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hEFA6A

Plasmid

#206412

Purpose

Mammalian expression of hEFA6A

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6A (PSD Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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FL-fibulin-3 pcDNA4

Plasmid

#29700

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk2b2

Plasmid

#13757

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEBG-Pak1 83-149 L107F

Plasmid

#12215

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Mammalian

Mutation

Autoinhibitory domain (aa 83-149) with L107F muta…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
NELFE-mOrange pHis-//

Plasmid

#53303

Purpose

Expresses His-tagged NELF-E-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

NELF-E protein (Nelf-E Fly)

Tags

His and mOrange

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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GST-BRD (1079-1198)

Plasmid

#99507

Purpose

Bacterial expression of mouse CBP bromodomain

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

mouse CBP BRD (1079-1198) (Crebbp Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG-F6C

Plasmid

#176546

Purpose

Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Tags

His tag

Expression

Bacterial

Mutation

change phenylalanine 6 to cysteine; change glutam…

Promoter

T5

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1202

Plasmid

#44484

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pOET3-N-TEV-Twin-Strep

Plasmid

#203323

Purpose

Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and Twin-strep-tag

Expression

Insect

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23119

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #4-RFP

Plasmid

#81078

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23107

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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MTK3a_007

Plasmid

#123760

Purpose

Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2b1

Plasmid

#13756

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b1 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pGP-CMV-NES-fRGECO1

Plasmid

#125247

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Tags

NES signal

Expression

Mammalian

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-dMASS_1

Plasmid

#209764

Purpose

Expression of dMASS_1 in mammalian cells under CMV promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Expression

Mammalian

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1001

Plasmid

#44476

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, [4-3] Gateway

Use

Gateway destination vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNEXdelta-ApAF

Plasmid

#13334

Depositor

Bong-Kiun Kaang

Article

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Insert

Aplysia activating factor

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
pLaNGo11-1

Plasmid

#126053

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-NRASshort

Plasmid

#13799

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

NRAS (NRAS Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pCFJ1002

Plasmid

#44477

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, [4-3] Gateway

Use

Gateway destination vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 18: RLuc.5’ATF4.GFP

Plasmid

#21864

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET28-sfGFP[D134TAG+N150TAA]

Plasmid

#164582

Purpose

superfolder GFP with D134TAG and N150TAA mutations

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-134TAG+150TAA

Tags

6xHis

Expression

Bacterial

Mutation

D134TAG + N150TAA Mutations

Promoter

T7

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

Plasmid

#52895

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pBstSK+IRES-V5-IPpoI (mutant)

Plasmid

#206322

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag-mutated IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein (mutant version)

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31YG121VDHFR_WTTS

Plasmid

#91229

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_G121VDHFR_WTTS

Plasmid

#91233

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4 mIL-17 1.1kb promoter

Plasmid

#20125

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/3xmyc_NLRP11-LRR

Plasmid

#131200

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

LRR of NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

contains only the leucine-rich repeats (LRRs), am…

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BDS-2 3x MUT (p53 binding site)

Plasmid

#16516

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant p53 binding sites

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ907

Plasmid

#44473

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, [4-3] Gateway

Use

Gateway destination vector

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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We narrowed to 7,325 results for: 11