Skip to main content
Addgene
Showing: 2751 - 2800 of 9181 results

  12. relAE335Q_NS1

    Plasmid
    #100917
    Purpose
    expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome
    Depositor
    Erin O'Shea
    Article
    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.
    Insert
    RelA fragment from E.coli with point mutation E335Q
    Use
    Cyanobacteria cloning vector
    Tags
    FLAG
    Expression
    Mutation
    E 335 converted to Q
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    100917-plasmid-map-sequence-id-196978

    Enlarge

  13. pECT1B

    Plasmid
    #75447
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75447-plasmid-map-sequence-id-146475

    Enlarge

  14. pECT1D

    Plasmid
    #75448
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75448-plasmid-map-sequence-id-146476

    Enlarge

  15. pECT1E

    Plasmid
    #75449
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1E
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75449-plasmid-map-sequence-id-146477

    Enlarge

  17. pKS013

    Plasmid
    #65466
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry
    Depositor
    Keith Tyo
    Article
    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pl-TetO
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pST50Trc4-HPCNDHFR

    Plasmid
    #63998
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63998-plasmid-map-sequence-id-177404

    Enlarge

  19. pST50Trc4-CBPNDHFR

    Plasmid
    #63995
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63995-plasmid-map-sequence-id-177401

    Enlarge

  20. pST50Trc4-HPCDHFR

    Plasmid
    #63993
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63993-plasmid-map-sequence-id-177399

    Enlarge

  21. pST50Trc3-DHFRCYD

    Plasmid
    #63988
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63988-plasmid-map-sequence-id-177394

    Enlarge

  22. pST50Trc3-HPCDHFR

    Plasmid
    #63975
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63975-plasmid-map-sequence-id-177381

    Enlarge

  23. pST50Trc3-HPCHISNDHFR

    Plasmid
    #63984
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63984-plasmid-map-sequence-id-177534

    Enlarge

  24. pST50Trc2-HPCNDHFR

    Plasmid
    #63962
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63962-plasmid-map-sequence-id-177368

    Enlarge

  25. pST50Trc3-FLAGDHFR

    Plasmid
    #63974
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63974-plasmid-map-sequence-id-177380

    Enlarge

  26. pST50Trc1-HPCHISNDHFR

    Plasmid
    #63948
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63948-plasmid-map-sequence-id-177464

    Enlarge

  27. pST50Trc1-CBPHISNDHFR

    Plasmid
    #63945
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Calmodulin Binding Peptide (CBP); TEV…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63945-plasmid-map-sequence-id-177351

    Enlarge

  28. pST50Trc4-STRDHFR

    Plasmid
    #63991
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63991-plasmid-map-sequence-id-177397

    Enlarge

  29. pST50Trc3-STRHISNDHFR

    Plasmid
    #63982
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63982-plasmid-map-sequence-id-177388

    Enlarge

  30. pST50Trc3-HPCNDHFR

    Plasmid
    #63980
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63980-plasmid-map-sequence-id-177386

    Enlarge

  31. pST50Trc3-CBPNDHFR

    Plasmid
    #63977
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63977-plasmid-map-sequence-id-177383

    Enlarge

  32. pST50Trc2-DHFRCYD

    Plasmid
    #63970
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63970-plasmid-map-sequence-id-177376

    Enlarge

  33. pST50Trc3-FLAGHISNDHFR

    Plasmid
    #63983
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63983-plasmid-map-sequence-id-177389

    Enlarge

  34. pST50Trc2-STRHISNDHFR

    Plasmid
    #63964
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63964-plasmid-map-sequence-id-177370

    Enlarge

  35. pST50Trc2-CBPHISNDHFR

    Plasmid
    #63963
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Calmodulin Binding Peptide (CBP); TEV…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63963-plasmid-map-sequence-id-177494

    Enlarge

  36. pST50Trc2-HPCDHFR

    Plasmid
    #63957
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63957-plasmid-map-sequence-id-177363

    Enlarge

  37. pST50Trc1-HPCNDHFR

    Plasmid
    #63944
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63944-plasmid-map-sequence-id-177350

    Enlarge

  38. pST50Trc1-DHFRCYD

    Plasmid
    #63952
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63952-plasmid-map-sequence-id-177358

    Enlarge

  39. pST50Trc1-HPCDHFR

    Plasmid
    #63939
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63939-plasmid-map-sequence-id-177345

    Enlarge

  41. pHL 342

    Plasmid
    #53012
    Purpose
    PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    mCherry
    araC
    ompC
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -81 to +36 relative to start codon
    Promoter
    Plar, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. pKB234

    Plasmid
    #170021
    Purpose
    Encodes LacI-controlled expression of surface-displayed hBD3 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    hBD3
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170021-plasmid-map-sequence-id-335964

    Enlarge

  47. pKB234.1

    Plasmid
    #170022
    Purpose
    Encodes LacI-controlled expression of surface-displayed hBD3 alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    hBD3 alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170022-plasmid-map-sequence-id-334667

    Enlarge

  48. pKB236

    Plasmid
    #170027
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    TCP
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170027-plasmid-map-sequence-id-335297

    Enlarge

Showing: 2751 - 2800 of 9181 results