We narrowed to 4,185 results for: Gca

1. pET28-sfGFP-SpyTag003 Loop A

   Plasmid

   #171776

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues V…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1344

   Plasmid

   #119273

   Purpose

   knockdown folA in E. aerogenes

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA folA (E. aerogenes)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GluR2_adRNA(2,100,50)-GFP

   Plasmid

   #124622

   Purpose

   Expresses adRNA(2,100,50) targeting the RAB7A transcript along with GFP

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   GluR2_adRNA(2,100,50)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GluR2_adRNA(0,100,50)-GFP

   Plasmid

   #124623

   Purpose

   Expresses adRNA(0,100,50) targeting the RAB7A transcript along with GFP

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   GluR2_adRNA(0,100,50)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJG493

   Plasmid

   #91197

   Purpose

   WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gUbi1+gUbi8+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMP-MBD2_3

   Plasmid

   #36370

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MBD2 (MBD2 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. gh127

   Plasmid

   #106797

   Purpose

   expression of gRNA targeting XYLT1

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   XYLT1 (XYLT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_HLF_2

   Plasmid

   #86302

   Purpose

   Encodes gRNA for 3' target of human HLF

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against HLF (HLF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-lpoB

   Plasmid

   #89959

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   lpoB gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ADEPT-pTarget-GFP

    Plasmid

    #238041

    Purpose

    Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

    Depositor

    Lingchong You

    Article

    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. ADEPT-pTarget-RFP

    Plasmid

    #238040

    Purpose

    Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

    Depositor

    Lingchong You

    Article

    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Lux

    Available since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. esgRNA_NbATML1-1pro

    Plasmid

    #231151

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbATML1-1pro
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PCNT

    Plasmid

    #227284

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of PCNT (PCNT Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDB_118

    Plasmid

    #216089

    Purpose

    Cas9 [Sp] CRISPRa targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210736

    Purpose

    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Merry sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1114H

    Plasmid

    #200640

    Purpose

    An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

    Depositor

    Omar Akbari

    Article

    Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

    Insert

    gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAT58

    Plasmid

    #204304

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJAT71

    Plasmid

    #204305

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pW319-lenti-sg3-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189949

    Purpose

    Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg3-Itga9) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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