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pcDNA3.1-HA-GTSE1-S91A

Plasmid

#224721

Purpose

For expression of HA-tagged GTSE1 protein with S91A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 91 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S157A

Plasmid

#224722

Purpose

For expression of HA-tagged GTSE1 protein with S157A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 157 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Use

Tags

His and V5

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_C942S_pCDNA3.1(+)

Plasmid

#187729

Purpose

Express the catalytically inactive NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Cys874 mutated to a serine

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_FL_pCDNA3.1(+)

Plasmid

#187730

Purpose

Express NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-malat1

Plasmid

#210580

Purpose

Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-malat1

Plasmid

#210581

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) M-Ras(G22C)

Plasmid

#224288

Purpose

Cell transfection and expression of M-Ras (G22C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

M-Ras (MRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G22C

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Rheb(R15C)

Plasmid

#224290

Purpose

Cell transfection and expression of Rheb (R15C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rheb (RHEB Human)

Use

Tags

Expression

Mammalian

Mutation

R15C

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) R-Ras1(G38C)

Plasmid

#224286

Purpose

Cell transfection and expression of R-Ras1 (G38C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

R-Ras1 (RRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G38C

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1:mTRESK(S116C)

Plasmid

#224772

Purpose

In vitro transcription for Oocyte expression of mTRESK(S116C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 18 (Kcnk18 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S116C

Promoter

T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rap1A(WT)

Plasmid

#224276

Purpose

Cell transfection and expression of Rap1A

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-RalA(WT)

Plasmid

#224274

Purpose

Cell transfection and expression of RalA

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

RalA (RALA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2(S156C)

Plasmid

#224770

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S156C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrascomQ61R

Plasmid

#206845

Purpose

To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Codons altered to most optimum based on mouse cod…

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-H2-18 Light chain

Plasmid

#216308

Purpose

Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-DCP1

Plasmid

#215843

Purpose

Expression of FLAG/HA-DCP1

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human DCP1 (DCP1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-nCas9n

Plasmid

#217487

Purpose

Expression of nuclear nCas9n

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-p54

Plasmid

#215844

Purpose

Expression of FLAG/HA-p54

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human p54

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ARL16-His6

Plasmid

#216484

Purpose

Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag

Depositor

Richard Kahn

Insert

ADP-ribosylation factor like 16 (ARL16 Human)

Use

Tags

includes GSGSGSHHHHHH (His6) tag at C-terminus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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