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pDD382

Plasmid

#91830

Purpose

Lox2272-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox2272-SEC-Lox2272

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB6574

Plasmid

#106132

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBT264

Plasmid

#27438

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

TRE-tdTomato-myc flanked by insulators

Use

Mouse Targeting; Tta dependent

Tags

Myc

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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Degron-KI p53 donor vector

Plasmid

#65619

Purpose

Donor vector to generate Degron-KI DD-p53

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

p53 Degron-KI left and right HR arms

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only
pATF4-donor

Plasmid

#113805

Purpose

CRISPR donor plasmid to tag human transcription factor ATF4 with GFP

Depositor

Kevin White

Insert

ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)

Use

CRISPR

Mutation

rs140428747

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmFMR1_B

Plasmid

#145920

Purpose

Insect Expression of DmFMR1

Depositor

Elisa Izaurralde

Insert

DmFMR1 (Fmr1 Fly)

Expression

Insect

Mutation

Two amino acid mutations (T214I and N501S) and th…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB4787

Plasmid

#106147

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-GFP osTIR1

Plasmid

#182514

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

GFP

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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