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HA-MBP-TEVpCS-ADGRF1 CTF-FLAG

Plasmid

#217694

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF1 (ADGRF1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-566

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD2 CTF-FLAG

Plasmid

#217688

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRD2 (ADGRD2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-636

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD1 CTF-FLAG

Plasmid

#217687

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRD1 (ADGRD1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-544

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF5 CTF-FLAG

Plasmid

#217698

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF5 (ADGRF5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-990

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG1 CTF-FLAG

Plasmid

#217699

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG1 (ADGRG1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-382

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(T59/T61A)(HA)

Plasmid

#195027

Purpose

HA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with T59/T61A mutation) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T59/T61A

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(T59/T61D)(HA)

Plasmid

#195028

Purpose

HA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T59/T61D

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m-ΔZF

Plasmid

#192276

Purpose

Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

Cas12m ΔZF

Use

CRISPR

Expression

Bacterial

Mutation

H459A, C552A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m-ΔZF

Plasmid

#192277

Purpose

pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m ΔZF

Use

CRISPR

Expression

Bacterial

Mutation

D485A, H549A, C552A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1356-1561_V

Plasmid

#147778

Purpose

Bacterial Expression of HsNot1iso1_1356-1561

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1356-1561 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1356-1628_V

Plasmid

#147781

Purpose

Bacterial Expression of HsNot1iso1_1356-1628

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1356-1628 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_1-234_AE

Plasmid

#148561

Purpose

Bacterial Expression of Hs4EHP_1-234

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234 (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_1-234-I85AM101A_AE

Plasmid

#148562

Purpose

Bacterial Expression of Hs4EHP_1-234-I85AM101A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234-I85AM101A (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_1-234-W95A_AE

Plasmid

#148563

Purpose

Bacterial Expression of Hs4EHP_1-234-W95A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234-W95A (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE

Plasmid

#148565

Purpose

Bacterial Expression of Hs4EHP_52-234opt-R103LE149L

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt-R103LE149L (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-Hs4EHP_52-234opt-Q159SM161DR202E_AE

Plasmid

#148567

Purpose

Bacterial Expression of Hs4EHP_52-234opt-Q159SM161DR202E

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt-Q159SM161DR202E (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-Left TALE

Plasmid

#196504

Purpose

Expresses left-side TALE–DddAtox N term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 left TALE-G1397 DddA-C-UGI-SV40 polyA

Expression

Bacterial

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND2-FusXTBE-Left TALE

Plasmid

#196498

Purpose

Expresses left-side TALE-DddAtox Cterm half ND2-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND2 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND2-FusXTBE-Right TALE

Plasmid

#196499

Purpose

Expresses Right-side TALE-DddAtox Nterm half ND2-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND2 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-Left TALE

Plasmid

#196500

Purpose

Expresses left-side TALE-DddAtox Cterm half Cox1-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-Cox1 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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