Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 11,356 results for: AGA

Showing: 11201 - 11220 of 11356 results

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Lenti-N1303K-ngRNA+14_EF1a-puroR

Plasmid

#207359

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR +14 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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px552-U6-gRNA1-CMV-eGFP

Plasmid

#211758

Purpose

sgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

Depositor

Glenn Yiu

Article

Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

gRNA1 targeting VEGF-A (VEGFA Mouse, Human, M. mulatta (rhesus macaque))

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E3(48))-PGKpuro2ABFP-W

Plasmid

#200483

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E3(48) (CXCR4 Human)

Use

Lentiviral

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BCL11B-E3(34))-PGKpuro2ABFP-W

Plasmid

#208558

Purpose

Lentiviral vector expressing gRNA targeting human BCL11B-E3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B-E3(34) (BCL11B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E4(59))-PGKpuro2AmCherry-W

Plasmid

#210615

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E4(59) (CXCR4 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E4(59))-PGKpuro2ABFP-W

Plasmid

#200485

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E4(59) (CXCR4 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

Plasmid

#209199

Purpose

Mutagenesis of Kcnma1

Depositor

Larry Zweifel

Article

Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

Insert

Kcnma1 (Kcnma1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO shLuc/FLAG Metap2

Plasmid

#209316

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 human, Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/LYCHOS WT-FLAG

Plasmid

#209315

Purpose

Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/FLAG Metap2

Plasmid

#209314

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-4gRNA-GBX2-RFP

Plasmid

#192288

Purpose

Expresses RFP and four sgRNAs against GBX2

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

RFP and four sgRNA GBX2 (GBX2 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209069

Purpose

Entry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209068

Purpose

Entry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD R1398A R1403A R1404A

Plasmid

#188134

Purpose

Expresses C-terminal flag-tagged human CAD R1398A R1403A R1404A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD R1403A R1404A

Plasmid

#188135

Purpose

Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD L1405A

Plasmid

#188136

Purpose

Expresses C-terminal flag-tagged human CAD L1405A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD A1400E

Plasmid

#188137

Purpose

Expresses C-terminal flag-tagged human CAD A1400E in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD C1374S

Plasmid

#188139

Purpose

Expresses C-terminal flag-tagged human CAD C1374S in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD E1367A

Plasmid

#188140

Purpose

Expresses C-terminal flag-tagged human CAD E1367A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD E1373A

Plasmid

#188142

Purpose

Expresses C-terminal flag-tagged human CAD E1373A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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