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pDONR223_RAC1_p.T17N

Plasmid

#82179

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Mutation

T17N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TGFBR1_WT

Plasmid

#82237

Purpose

Gateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TGFBR1 (TGFB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

Plasmid

#156408

Purpose

pCS2 CMV expression vector driving EYFP fused human KRAS4B

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_BMP2_WT

Plasmid

#82101

Purpose

Gateway Donor vector containing BMP2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMP2 (BMP2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pZ M2rtTA_CAGG TetON-Sox10

Plasmid

#115240

Purpose

RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

SOX10 (SOX10 Human)

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GqDREADD-dTomato-Fishell-4

Plasmid

#83897

Purpose

Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM3Dq

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-NES-OCaMP-WPRE

Plasmid

#224934

Purpose

pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSynI

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

Plasmid

#216152

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERN1_WT

Plasmid

#82115

Purpose

Gateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERN1 (ERN1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SHC1_WT_V5

Plasmid

#82931

Purpose

Gateway Donor vector containing SHC1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SHC1 (SHC1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CD79B_WT

Plasmid

#81750

Purpose

Gateway Donor vector containing CD79B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CD79B (CD79B Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-ChR2-mCherry-Fishell-3

Plasmid

#83898

Purpose

ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

ChR2

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT3_WT

Plasmid

#82235

Purpose

Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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U6-PGK-hCD2

Plasmid

#224294

Purpose

Expresses sgRNA from U6 promoter and human CD2 under PGK

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

U6 sgRNA cassette with CD2 under PGK promoter (CD2 Human)

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6/PGK

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLX313-TP53-WT

Plasmid

#118014

Purpose

Constitutive expression of wild-type human Tumor Protein p53 (TP53)

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GiDREADD-dTomato-Fishell-5

Plasmid

#83896

Purpose

Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM4Di

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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