We narrowed to 19,295 results for: CAL

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pTWIN1-His6-Ssp-Htt2-90-49Q-P90C

Plasmid

#114631

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 49 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

Plasmid

#114611

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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FBXO5 D3.4 gRNA

Plasmid

#90686

Purpose

3rd generation lentiviral gRNA plasmid targeting human FBXO5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FBXO5 (Guide Designation D3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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SMC6 G4.4 gRNA

Plasmid

#90899

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC6 (Guide Designation G4.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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ASPM A5.2 gRNA

Plasmid

#90537

Purpose

3rd generation lentiviral gRNA plasmid targeting human ASPM

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ASPM (Guide Designation A5.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSV272-PfMBP-HypDAE

Plasmid

#110296

Purpose

Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase Activating Enzyme

Tags

His6 and Maltose Binding Protein

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJO428

Plasmid

#111141

Purpose

URT-type vector for Val-mIκBα expression in Mammalian cells

Depositor

Alexander Varshavsky

Article

Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM2-mRUBY2

Plasmid

#107793

Purpose

donor vector for knock-in gene editing at human DNM2 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM2-mRuby2 (DNM2 Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Expression

Bacterial and Yeast

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S-HLx8

Plasmid

#108138

Purpose

Recruitable inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mECitrine

Plasmid

#105153

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

Insert

kanMX6-P81nmt1-mECitrine

Tags

81nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

81nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis PL-T500

Plasmid

#92314

Purpose

Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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NDEL1 F4.2 gRNA

Plasmid

#90792

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDEL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDEL1 (Guide Designation F4.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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ACTL6A C8.3 gRNA

Plasmid

#90521

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACTL6A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ACTL6A (Guide Designation C8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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RNASEH2A B9.3 gRNA

Plasmid

#90878

Purpose

3rd generation lentiviral gRNA plasmid targeting human RNASEH2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RNASEH2A (Guide Designation B9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PSMD14 E6.4 gRNA

Plasmid

#90859

Purpose

3rd generation lentiviral gRNA plasmid targeting human PSMD14

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PSMD14 (Guide Designation E6.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PSMD14 E5.4 gRNA

Plasmid

#90858

Purpose

3rd generation lentiviral gRNA plasmid targeting human PSMD14

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PSMD14 (Guide Designation E5.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PRC1 G2.2 gRNA

Plasmid

#90857

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PRC1 (Guide Designation G2.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PRC1 G1.2 gRNA

Plasmid

#90856

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PRC1 (Guide Designation G1.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PPP2R5E C1.5 gRNA

Plasmid

#90855

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R5E

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R5E (Guide Designation C1.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PPP2R2C B7.5 gRNA

Plasmid

#90849

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R2C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R2C (Guide Designation B7.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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