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pDONR223_PIK3CG_p.K973R

Plasmid

#81628

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway Cloning

Mutation

E2G; K973R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CG_p.W212C

Plasmid

#81630

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway Cloning

Mutation

E2G; W212C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CG_p.V168M

Plasmid

#81542

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway Cloning

Mutation

E2G; V168M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.D44N

Plasmid

#81456

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NM_005163.2 (AKT1 Human)

Use

Gateway Cloning

Mutation

D44N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.E268D

Plasmid

#81403

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway Cloning

Mutation

E268D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.G137E

Plasmid

#81407

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway Cloning

Mutation

G137E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ACTG1_p.K61E

Plasmid

#81397

Purpose

Gateway Donor vector containing ACTG1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ACTG1 (ACTG1 Human)

Use

Gateway Cloning

Mutation

K61E; H88Y; p.374_375delCF-insSS

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTU2_2a

Plasmid

#73010

Purpose

Secondary module (BpiI) site in pTU2a backbone

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Secondary module + Golden Gate pTU2a BsmBI destination site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pet28bZFCNGA5

Plasmid

#69858

Purpose

His tagged protein for antibody production screening

Depositor

Gary Matthews

Article

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTRmut

Plasmid

#63894

Purpose

Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pSG 415

Plasmid

#63862

Purpose

Detect translation initiation from AUC start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUC start codon

Promoter

PtacI

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pSG 414

Plasmid

#63861

Purpose

Detect translation initiation from UUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 431

Plasmid

#63866

Purpose

Detect translation initiation from GUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

GUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
psicheck-2-Casp8

Plasmid

#48172

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of CASP8 (CASP8 Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pEMS1431

Plasmid

#29226

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple77 (GABRA6 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1121

Plasmid

#29045

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple90 (GFAP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1180

Plasmid

#29092

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple84 (GAL Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1434

Plasmid

#29229

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple80 (GABRA6 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1433

Plasmid

#29228

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple79 (GABRA6 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1414

Plasmid

#29211

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple85 (GCHFR Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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