We narrowed to 17,160 results for: REV

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pRH3069

Plasmid

#210163

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151V-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151V-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151V

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3047

Plasmid

#210157

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151H-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151H-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151H

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3055

Plasmid

#210161

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151Q-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151Q-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151Q

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3048

Plasmid

#210158

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151Y-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151Y-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151Y

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3053

Plasmid

#210159

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151A

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Atg32 [200-341] 1B

Plasmid

#190431

Purpose

Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Atg32-deltaPsR pXP721

Plasmid

#200735

Purpose

Plasmid for the expression of GFP-Atg32 lacking the PsR domain in S. cerevisiae.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Tags

GFP

Expression

Yeast

Mutation

Amino acids 200-341 are deleted.

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3 1-66-EGFP

Plasmid

#201432

Purpose

Bacterial protein expression of Edc3 1-66

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-66 only

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-ScEap1p_80-122_AF

Plasmid

#148707

Purpose

Bacterial Expression of ScEap1p_80-122

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_80-122 (EAP1 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-SceIF4G_408-485_AF

Plasmid

#148713

Purpose

Bacterial Expression of SceIF4G_408-485

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4G_408-485 (TIF4631 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-SceIF4G_408-500_AF

Plasmid

#148714

Purpose

Bacterial Expression of SceIF4G_408-500

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4G_408-500 (TIF4631 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-ScEap1p_91-150_AF

Plasmid

#148710

Purpose

Bacterial Expression of ScEap1p_91-150

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_91-150 (EAP1 Budding Yeast)

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJL29

Plasmid

#174063

Purpose

Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc

Tags

FLAG

Expression

Yeast

Promoter

pCyc1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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HCP4

Plasmid

#166106

Purpose

This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

Depositor

Matthias Heinemann

Insert

Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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HCP6

Plasmid

#166108

Purpose

This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

Depositor

Matthias Heinemann

Insert

Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3G919C

Plasmid

#120255

Purpose

Expression of ATP3-7 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Mutation

G919 changed to C; Alanine 307 changed to Proline

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 CUP2 (GB2716)

Plasmid

#160599

Purpose

Copper dependent transcription factor

Depositor

Diego Orzaez

Insert

CUP2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD217

Plasmid

#160939

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mTagBFP2-HA Tag

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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