167,150 results
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Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV.CMV.SV40.THBS1-HA.SV40(polyA)
Plasmid#154850PurposeExpresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminusDepositorAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcs2-GFP-IRES-mCherry
Plasmid#226098PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST
Plasmid#208041PurposeEnables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidinDepositorInsertTurboID
UseLentiviralPromoterEFSAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
ExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GCaMP6f
Plasmid#67564Purposebacterial expression of a fluorescent calcium reporterDepositorInsertGCaMP6f
TagsHIS-tagExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RB
Plasmid#60522PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 eta
Plasmid#116887PurposeExpresses HA-tagged human 14-3-3 eta in mammalian cellsDepositorAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_Ado1.0mut
Plasmid#153290PurposeExpress the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytesDepositorInsertAdenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
UseAAVPromoterGfaABC1DAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only