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I-TCTC

Plasmid

#43184

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-TCTC

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
modRNAc1 2x k-turn CLTX msCD28-CD3z CAR

Plasmid

#213984

Purpose

Chlorotoxin (CLTX)-CAR msCD3z gene in modRNA cap1 vector

Depositor

Xiaoping Bao

Article

Chang et al Nat Biomed Eng. 2026 Apr 24:10.1038/s415

Insert

CLTC CAR

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pUltra BCR-ABL V468F

Plasmid

#210435

Purpose

Lentiviral expression of BCR-ABL V468F

Depositor

Justin Pritchard

Article

Sokirniy et al Genome Med. 2026

Insert

BCR-ABL (ABL1, BCR Human)

Use

Lentiviral

Tags

EGFP-P2A

Mutation

V468F

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pFRT-B-RPCNA (pc1205)

Plasmid

#247000

Purpose

This pFRT-B plasmid enables the creation of a stable mammalian cell line expressing RFP-tagged PCNA .

Depositor

Cristina Cardoso

Article

Chagin et al Nat Commun. 2016 Apr 7;7:11231. doi: 10.

Insert

PCNA (PCNA Human)

Use

Cre/Lox

Tags

RFP

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFRT-B-GPCNA (pc1274)

Plasmid

#247001

Purpose

This pFRT-B plasmid enables the creation of a stable mammalian cell line expressing GFP-tagged PCNA

Depositor

Cristina Cardoso

Article

Chagin et al Nat Commun. 2016 Apr 7;7:11231. doi: 10.

Insert

PCNA (PCNA Human)

Use

Cre/Lox

Tags

GFP

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pRS316-pTEF-Sc_IntAct-tCYC

Plasmid

#248980

Purpose

Low-copy centromeric plasmids which expresses Saccharomyces cerevisiae IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)

Depositor

Saravanan Palani

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Sc_IntAct

Tags

ALFA tag

Expression

Yeast

Promoter

TEF

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pDUAL-pAct1-Sp_IntAct-tADH1

Plasmid

#250862

Purpose

Low-copy centromeric plasmids which expresses Schizosaccharomyces pombe IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)

Depositor

Saravanan Palani

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Sp_IntAct

Tags

ALFA tag

Expression

Yeast

Promoter

TEF

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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DDR1-P1

Plasmid

#236327

Purpose

Study the role and mechanism of the 3' UTR in regulating DDR1-P1 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

DDR1-P1 3'UTR (DDR1 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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DDR1-P2

Plasmid

#236328

Purpose

Study the role and mechanism of the 3' UTR in regulating DDR1-P2 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

DDR1-P2 3'UTR (DDR1 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-eNme2.C.NR-nCas9(D16A)-PolI5MΔ

Plasmid

#249077

Purpose

Expresses nucleus-localized eNme2.C.NR-nCas9 (D16A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

eNme2.C.NR-nCas9-PolI5MΔ

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

deletion of PolI5M N-terminal flap endonuclease d…

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-H4-BioID2-HA

Plasmid

#232026

Purpose

mammalian expression (CMV promoter) of histone H4 fused to BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

H4 (H4C14 Human)

Tags

BioID2-HA

Expression

Mammalian

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mTDGa.2-BioID2-HA

Plasmid

#232029

Purpose

mammalian expression (CMV promoter) of TDGa.2 (SIM mutant E321Q) fused to BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

Tdg (Tdg Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

K341A (site directed mutagenesis: aag -> aag)

Promoter

CMV

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG coca-5HT3-IRES-GFP

Plasmid

#242193

Purpose

Expression vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

coca-5HT3-IRES-GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

CAG

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hIRF5-V5(4D)-LPETG

Plasmid

#237225

Purpose

Expresses LPETG-tagged, constitutively active human IRF5 variant in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Interferon regulatory factor 5 (IRF5 Human)

Tags

6xHis

Expression

Bacterial

Mutation

S451D, S453D, S456D, S462D

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Plasmid

#238314

Purpose

ScVip1-536-1107-delta848-918-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E576A-K623A-Q625S-K627A

Plasmid

#238327

Purpose

ScVip1-536-1107-E576A-K623A-Q625S-K627A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E576A-K623A-Q625S-K627A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_2b)-PGKpuro2ABFP-W

Plasmid

#208415

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_1k)-PGKpuro2ABFP-W

Plasmid

#208414

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K558A-K605A-K732A-K817A

Plasmid

#238325

Purpose

ScVip1-536-1107-K558A-K605A-K732A-K817A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K558A-K605A-K732A-K817A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)

Plasmid

#233270

Purpose

To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

Depositor

Jonathan Ploski, Glenn Schafe

Article

Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

Insert

GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)

Use

AAV

Tags

GFP

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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