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KIF5C(1-560)-EF(C)

Plasmid

#61675

Purpose

Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-EF(C) (Kif5c Rat)

Expression

Mammalian

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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LI B-C N-NES KOZ b c7

Plasmid

#54445

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear Export Signal (NES) of heat stable inhibitor (PKI)

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEmerald-SpHis5

Plasmid

#44851

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Emerald with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEmerald

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-SpHis5

Plasmid

#44840

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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p70rg 5

Plasmid

#17829

Depositor

Jay Keasling

Publication

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
mCherry-NovoSplit657(B)

Plasmid

#255882

Purpose

Expression plasmid for NovoSplit657(B), the second part of the NovoSplit system, fused to mCherry for fluorescence labeling.

Depositor

Julia Mahamid

Publication

Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1

Insert

NovoSplit657(B)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pOS1T-MmPylRS(7_1)

Plasmid

#253669

Purpose

Production of engineered aaRS/tRNA pair for incorporation of 3ThA

Depositor

Alexandria Deliz Liang

Publication

Natter Perdiguero et al J Am Chem Soc. 2026 Apr 8;148(13):13619-

Inserts

MmPylRS(7_1)
MsPylT_CUA

Expression

Bacterial

Available since

June 25, 2026

Availability

Academic Institutions and Nonprofits only

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pOS1T-MmPylRS(8_2)

Plasmid

#253670

Purpose

Production of engineered aaRS/tRNA pair for incorporation of 3PyA

Depositor

Alexandria Deliz Liang

Publication

Natter Perdiguero et al J Am Chem Soc. 2026 Apr 8;148(13):13619-

Inserts

MmPylRS(8_2)
MsPylT_CUA

Expression

Bacterial

Available since

June 25, 2026

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-EcYRS-4xYtR-ITR-GFP

Plasmid

#240279

Purpose

Express AAV2 transgene (EGFP) and EcYRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Publication

Pham et al Angew Chem Int Ed Engl. 2026 Jan 1:e1615

Inserts

AAV2-ITR-EGFP
EcYRS
EcYtR

Use

AAV

Expression

Mammalian

Available since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_GFAP_SypHer3s_cyto

Plasmid

#238969

Purpose

Mammalian astrocytes SypHer3s expression

Depositor

Dmitry Bilan

Publication

Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

Insert

SypHer3s

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMT451

Plasmid

#139391

Purpose

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Publication

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)

Use

Synthetic Biology

Promoter

BA inducible promoters

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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UAST-uMCas12a+nls2x

Plasmid

#230909

Purpose

inducible expression of Cas12a+ with Gal4.

Depositor

Michael Boutros

Publication

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

Cas12a+

Use

CRISPR

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Publication

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEutRwc

Plasmid

#216334

Purpose

An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

Depositor

Mark Styczynski

Publication

McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

Inserts

EutR
Superfolder green fluorescent protein

Expression

Bacterial

Available since

Dec. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAM232

Plasmid

#226654

Purpose

pET24b-p97-FLAG

Depositor

Andreas Martin

Publication

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

FLAG

Expression

Bacterial

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-hTRPT1-iso1

Plasmid

#184239

Purpose

For purification of N-terminally His-tagged TRPT1 from bacteria

Depositor

Karla Feijs

Publication

Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

Insert

TRPT1 (TRPT1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

ICAM2 promoter

Expression

Mammalian

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-TAEL (JDW 1324)

Plasmid

#224508

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-H2A_mCherry_SV40pA (JDW 967)

Plasmid

#224528

Purpose

A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2A-mCherry-SV40pA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pROSA26-SAT

Plasmid

#208339

Purpose

ROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT signaling

Depositor

Philippe Soriano

Publication

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SAT sensor

Use

Mouse Targeting

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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