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pDESTFLAGHApuro3-Ago2Q545A

Plasmid

#14101

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Gln545 to Ala

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2K533AQ545A

Plasmid

#14103

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Gln545 to Alanine

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf148

Plasmid

#12836

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf148

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf72

Plasmid

#12760

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

A34R

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf263

Plasmid

#12951

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf263

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pAG147 BC072452 3'UTR

Plasmid

#12005

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N12 3'UTR (FAM107B Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG228 TNKS2 3'UTR mut

Plasmid

#12031

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

TNKS2 3'UTR mut (TNKS2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG174 TNS3 3'UTR mut

Plasmid

#12032

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N7 3'UTR mut (TNS3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG219 RIPK4 3'UTR mut

Plasmid

#12049

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N20 3'UTR mut (RIPK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG72 PFTK1 3'UTR

Plasmid

#12028

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

PFTK1 3'UTR (CDK14 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG87 DUXAP6 3'UTR mut

Plasmid

#12060

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N6 3'UTR mut (DUXAP6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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FLAG-SpEAF 60-251 pBacPAK8

Plasmid

#15321

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

EAF1 (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Mutation

N terminal deletion lacking first 59 amino acids …

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf82

Plasmid

#12770

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf82

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pBS Calponin3

Plasmid

#19194

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Calponin 3 (CNN3 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf152

Plasmid

#12840

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf152

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
p028g13-Gb1

Plasmid

#249632

Purpose

Clone 8xHis-residues in tandem with Gb1 polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3

Depositor

Isabel Abreu

Article

Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi

Insert

N-terminal tag with 8x His-residues in tandem with Gb1 polypeptide

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCL1054

Plasmid

#252438

Purpose

HAT1 tagged on the N-terminus with TEV-cleavable eGFP and selection with puromycin

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

HAT1 (HAT1 Human)

Tags

EGFP-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0950

Plasmid

#252418

Purpose

KPNB1 tagged on the N-terminus with mCherry.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

KPNB1 (KPNB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac-WT-REPAIR

Plasmid

#252296

Purpose

Expresses REPAIR (WT) in insect cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR (WT)

Expression

Insect

Mutation

dPspCas13b H133A/H1058A; ADAR2DD E488Q

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAcBAc1-Ma-tfmW-RSA2

Plasmid

#251519

Purpose

Orthogonal Methanomethylophilus alvus aaRS/tRNA-mediated incorporation of tfm-Tryptophan into sfGFP150TAG in HEK293T cells

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

Ma-tfmW-RSA2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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