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MAP4K2 gRNA (BRDN0001144728)

Plasmid

#76779

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K2 (MAP4K2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K2 gRNA (BRDN0001146560)

Plasmid

#76780

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K2 (MAP4K2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαWT-IRES-EGFP

Plasmid

#183456

Purpose

Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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CAMKK2 gRNA (BRDN0001145732)

Plasmid

#76696

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMKK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMKK2 (CAMKK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594E

Plasmid

#131714

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594E

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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TGFBR2 gRNA (BRDN0001146812)

Plasmid

#77468

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGFBR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TGFBR2 (TGFBR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001149482)

Plasmid

#76623

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1-CS2

Plasmid

#52367

Purpose

expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R95Q and S194A (according to numbering in Fig. 2A…

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
DYRK1B gRNA (BRDN0001149418)

Plasmid

#76621

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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GRK5 gRNA (BRDN0001149126)

Plasmid

#76271

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK5 (GRK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK1 gRNA (BRDN0001145955)

Plasmid

#77476

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK1 (CAMK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB4 gRNA (BRDN0001144879)

Plasmid

#76352

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB4 (EPHB4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB4 gRNA (BRDN0001148436)

Plasmid

#76353

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB4 (EPHB4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry IL36RN S113L

Plasmid

#58321

Purpose

entry vector for human IL36RN S113L mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Mutation

S113L: c.338C>T, p.Ser113Leu

Promoter

CMV

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
MARK1 gRNA (BRDN0001149474)

Plasmid

#77793

Purpose

3rd generation lentiviral gRNA plasmid targeting human MARK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MARK1 (MARK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001148884)

Plasmid

#77194

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TWF1 gRNA (BRDN0001149022)

Plasmid

#77287

Purpose

3rd generation lentiviral gRNA plasmid targeting human TWF1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TWF1 (TWF1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TWF1 gRNA (BRDN0001147784)

Plasmid

#77288

Purpose

3rd generation lentiviral gRNA plasmid targeting human TWF1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TWF1 (TWF1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP4K2 gRNA (BRDN0001148469)

Plasmid

#76781

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K2 (MAP4K2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge