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Showing: 1681 - 1710 of 5454 results

  4. INT_GFPsp

    Plasmid
    #212144
    Purpose
    Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23119
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212144-plasmid-map-sequence-id-420757

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  5. INT_GFPsp_CamRcargo

    Plasmid
    #212143
    Purpose
    Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23119
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212143-plasmid-map-sequence-id-420641

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  9. pKB237.1

    Plasmid
    #170031
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL19 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170031-plasmid-map-sequence-id-336779

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  10. pKB235.2

    Plasmid
    #170025
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL20 alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170025-plasmid-map-sequence-id-336784

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  11. pKB237.2

    Plasmid
    #170032
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL19 alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170032-plasmid-map-sequence-id-336778

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  13. pRB30-GFP

    Plasmid
    #165481
    Purpose
    pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and His
    Depositor
    Renato Bruni
    Article
    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2
    Type
    Empty backbone
    Use
    Tags
    TEV-GFP-FLAG-10x His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    165481-plasmid-map-sequence-id-324824

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  20. pKS013

    Plasmid
    #65466
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry
    Depositor
    Keith Tyo
    Article
    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pl-TetO
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pST50Trc4-CBPNDHFR

    Plasmid
    #63995
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63995-plasmid-map-sequence-id-177401

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  24. pST50Trc3-HPCHISNDHFR

    Plasmid
    #63984
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63984-plasmid-map-sequence-id-177534

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  25. pST50Trc1-HPCHISNDHFR

    Plasmid
    #63948
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63948-plasmid-map-sequence-id-177464

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  26. pST50Trc1-CBPHISNDHFR

    Plasmid
    #63945
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Calmodulin Binding Peptide (CBP); TEV…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63945-plasmid-map-sequence-id-177351

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  27. pST50Trc3-STRHISNDHFR

    Plasmid
    #63982
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63982-plasmid-map-sequence-id-177388

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  28. pST50Trc3-CBPNDHFR

    Plasmid
    #63977
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63977-plasmid-map-sequence-id-177383

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  29. pST50Trc2-STRHISNDHFR

    Plasmid
    #63964
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63964-plasmid-map-sequence-id-177370

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  30. pST50Trc2-CBPHISNDHFR

    Plasmid
    #63963
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Calmodulin Binding Peptide (CBP); TEV…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63963-plasmid-map-sequence-id-177494

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Showing: 1681 - 1710 of 5454 results