We narrowed to 4,128 results for: pCMV mammalian

1. pCMV-Neo-Bam APC 1-1941

   Plasmid

   #16510

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Mutation

   Codon 1941 truncation

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Neo-Bam APC 1-2644

   Plasmid

   #16511

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Mutation

   Codon 2644 truncation

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-Neo-Bam APC 1-331

   Plasmid

   #16508

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Mutation

   Codon 331 truncation

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

4. 3XFlag pCMV5-TOPO TAZ (S89A)

   Plasmid

   #24815

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Serine 89 mutated to Alanine

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCMV-SNAP-CRY2-VHH(GFP)

   Plasmid

   #58370

   Purpose

   For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Insert

   CRY2[1-498] (CRY2 Mustard Weed)
   

   Tags

   SNAP and vhhGFP4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 3XFlag pCMV5-TOPO TAZ (∂304)

   Plasmid

   #24810

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Contains ONLY amino acids 1-304.

   Available Since

   June 3, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. 3XFlag pCMV5-TOPO TAZ (106∂)

   Plasmid

   #24814

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-106

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pCMV5 Flag-Smad1 (4SP/AP+AAVA)

   Plasmid

   #14955

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

   Insert

   Smad1 (4SP/AP+AAVA) (SMAD1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Four Serine to Alanine mutations in the linker re…

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-FLAG-WRAP53beta-siRNA-resistant

   Plasmid

   #64680

   Purpose

   expresses siRNA resistant WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   CMV

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 synthetic, Human)
    

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Sport6-CD81-pHuji

    Plasmid

    #130904

    Purpose

    Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHuji (CD81 Human, Synthetic)
    

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Leu-49 and Gly-50 in extra…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-Sport6-CD9-pHuji

    Plasmid

    #130906

    Purpose

    Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHuji (CD9 Human, Synthetic)
    

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Asn50 and Asn51 in extrace…

    Promoter

    CMV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMVtrunc msfGFPΔC10-Utr28-222

    Plasmid

    #231558

    Purpose

    Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Utrophin calponin homology domain (UTRN Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-hAR-L26A,F27A,W433A,L436A,F437A

    Plasmid

    #89085

    Purpose

    mammalian expression of human androgen receptor with mutations of N-terminal FxxLF and WxxLF motifs

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR FxxLF WxxLF mutant (AR Human)
    

    Expression

    Mammalian

    Mutation

    hAR-L26A,F27A,W433A,L436A,F437A

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-Myc-XPLN (delta)N (aa1-117 deleted)

    Plasmid

    #73382

    Purpose

    Expresses myc tagged XPLN in mammalian cells with deletion of N-terminal amino acids 1-117

    Depositor

    Jie Chen

    Article

    Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

    Insert

    arhgef3 (ARHGEF3 Human)
    

    Tags

    Myc (QKLISEEDL)

    Expression

    Mammalian

    Mutation

    Deleted N-terminal 1-117 aa from XPLN protein

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid

    #223087

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid

    #223079

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid

    #223102

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)

    Plasmid

    #223103

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)

    Plasmid

    #223104

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-T7-SpCas9(MRKSKC)-P2A-EGFP (RAS2840)

    Plasmid

    #223105

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKSKC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKSKC)=D1135M, S1136R, G1218K, E1219S, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-T7-SpCas9(KWKYES)-P2A-EGFP (RAS2900)

    Plasmid

    #223095

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWKYES amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWKYES)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWKYES)=D1135K, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-T7-SpCas9(LWKYEY)-P2A-EGFP (RAS3001)

    Plasmid

    #223096

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYEY amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYEY)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYEY)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-T7-SpCas9(MKACKV)-P2A-EGFP (AHK136)

    Plasmid

    #223097

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKACKV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MKACKV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MKACKV)=D1135M, S1136K, G1218A, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-T7-SpCas9(MKRCKS)-P2A-EGFP (AHK140)

    Plasmid

    #223098

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MKRCKS)=D1135M, S1136K, G1218R, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-T7-SpCas9(KRRCKV)-P2A-EGFP (RAS3014)

    Plasmid

    #223099

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KRRCKV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRRCKV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KRRCKV)=D1135K, S1136R, G1218R, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-T7-SpCas9(VKMAKG)-P2A-EGFP (RAS3015)

    Plasmid

    #223100

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with VKMAKG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(VKMAKG)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(VKMAKG)=D1135V, S1136K, G1218M, E1219A, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-T7-SpCas9(SKNCKS)-P2A-EGFP (RAS2951)

    Plasmid

    #223101

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SKNCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(SKNCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(SKNCKS)=D1135S, S1136K, G1218N, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-T7-SpCas9(KWAVIG)-P2A-EGFP (RAS2999)

    Plasmid

    #223088

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWAVIG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWAVIG)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWAVIG)=D1135K, S1136W, G1218A, E1219V, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-T7-SpCas9(MWRQLM)-P2A-EGFP (RAS2868)

    Plasmid

    #223089

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWRQLM amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MWRQLM)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MWRQLM)=D1135M, S1136W, G1218R, E1219Q, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-T7-SpCas9(KWHMLM)-P2A-EGFP (RAS2869)

    Plasmid

    #223090

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWHMLM amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWHMLM)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWHMLM)=D1135K, S1136W, G1218H, E1219M, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-T7-SpCas9(MRKCLN)-P2A-EGFP (AHK130)

    Plasmid

    #223091

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCLN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKCLN)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKCLN)=D1135M, S1136R, G1218K, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-T7-SpCas9(LWKFEG)-P2A-EGFP (RTW3931)

    Plasmid

    #223092

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKFEG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKFEG)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKFEG)=D1135L, S1136W, G1218K, E1219F, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-T7-SpCas9(CWNWNS)-P2A-EGFP (RAS3011)

    Plasmid

    #223093

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with CWNWNS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(CWNWNS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(CWNWNS)=D1135C, S1136W, G1218N, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)

    Plasmid

    #223094

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWRYEK)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWRYEK)=D1135L, S1136W, G1218R, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-T7-SpCas9(LWKYSS)-P2A-EGFP (RAS3024)

    Plasmid

    #223080

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYSS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYSS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYSS)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge