We narrowed to 3,845 results for: 28
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Plasmid#196483PurposeTo express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.DepositorInsertazidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
ExpressionMammalianPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2
Plasmid#182351PurposeGermline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo*
Plasmid#140159PurposeExpresses a regulatable secretory cargo (without an ER export signal) for mammalian cellsDepositorInsertpiGH-DsRed-Express2-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
Plasmid#114111Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
ExpressionMammalianPromoterCAGAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
Plasmid#182358PurposePositive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.DepositorInsertGFP
ExpressionWormPromoterPmex-5Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-CFP-STM2(15-746)
Plasmid#18863DepositorInsertStromal Interaction Molecule 2 (STIM2 Human)
TagsCFPExpressionMammalianMutationCFP inserted between signal peptide and residues …Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag PA1
Plasmid#15559DepositorAvailable SinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) WT
Plasmid#134695PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purificationDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2
Plasmid#182350PurposeUbiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LEP-shREN
Plasmid#105580Purposeretrovirally express control shRNA with puro resistance and GFP markerDepositorInsertcontrol shRNA targeting luciferase
UseRNAi and RetroviralExpressionMammalianPromoterMSCV-LTRAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196079PurposeConstitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
TagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB.FRB-3myc-KLC1a
Plasmid#134627PurposeMammalian expression of mouse KLC1aDepositorInsertKLC1a (Klc1 Mouse)
TagsFRB, 3mycExpressionMammalianMutationWTPromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC1765
Plasmid#141346PurposeMini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop B
Plasmid#171780PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-DogTag Loop B
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiReport SHIP1 3' UTR
Plasmid#26531DepositorAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2
Plasmid#182355PurposemScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only