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Plasmid#73622PurposeExpresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tagDepositorInsertAlkB (alkB E. coli)
UseTagsHisExpressionBacterialMutationdeletion of first 11 AAPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET20b-GpNLuc-6xHis
Plasmid#135932PurposeExpresses the GpNLuc LumiFluor in bacterial cells.DepositorInsertGpNLuc LumiFluor
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 (1-242)
Plasmid#24401DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 1-242 only.PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a- hPTP1e-PDZ2
Plasmid#20377DepositorInserthPTP1e PDZ2 (PTPN13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 A141S
Plasmid#16157DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationA141SPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-deltaPYD
Plasmid#51535PurposeExpress His6-tagged HIN domain of mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28 Set9 mutant
Plasmid#24083DepositorInsertSet9 (SETD7 Human)
UseTagsFLAG and HisExpressionBacterialMutationH297APromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
PET28-FnCas12a-EP16
Plasmid#183636PurposePET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.DepositorInsertFnCas12a-EP16
UseCRISPRTags6×His TagExpressionBacterialMutationN607R, K613V, N617R, K180S, K660R, D616NPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Lys13Arg
Plasmid#50726Purposeoverexpression of the human TPI1 Lys13Arg allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Lysine 13 to ArgeninePromoterT7Available sinceFeb. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TrmD_HaeIn/pET28b(+) N,C-tag
Plasmid#12665DepositorInserttRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GST-Q11
Plasmid#185790PurposeEncodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertGST-Q11
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Mouse 1-87)
Plasmid#62061PurposeExpression of Mouse p53 (residues 1-87) in e. coliDepositorInsertp53 (Trp53 Mouse)
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Pep-H
Plasmid#185782PurposeEncodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Pep-H
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A774L
Plasmid#174796PurposeBacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-A774L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA774L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-W318R
Plasmid#174793PurposeBacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)DepositorInsertPARP1-W318R (PARP1 Human)
UseTags6xHisExpressionBacterialMutationW318, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only